{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:46:09Z","timestamp":1785717969226,"version":"3.56.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013348","name":"Innosuisse - Schweizerische Agentur f\u00fcr Innovationsf\u00f6rderung","doi-asserted-by":"publisher","award":["25866.2 PFLS\u2010LS"],"award-info":[{"award-number":["25866.2 PFLS\u2010LS"]}],"id":[{"id":"10.13039\/501100013348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["407940_206399"],"award-info":[{"award-number":["407940_206399"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012545","name":"Foundation for Research in Science and the Humanities","doi-asserted-by":"publisher","award":["STEB\u20102022\u2010002"],"award-info":[{"award-number":["STEB\u20102022\u2010002"]}],"id":[{"id":"10.13039\/100012545","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Allergen immunotherapy (AIT) is the only curative treatment for allergic diseases, primarily by inducing allergen\u2010neutralising IgG antibodies. However, its use is limited by frequent allergic adverse events.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To facilitate the design of IgG\u2010epitope\u2010based peptide vaccines, this study aimed to identify IgG\u2010binding regions on the major birch pollen allergen,\n                      <jats:italic>Bet v 1<\/jats:italic>\n                      , using patient\u2010derived immune profiles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples were collected from 30 birch pollen\u2010allergic patients, both AIT\u2010treated and untreated; healthy individuals were included as controls. Sera were analysed for allergen\u2010specific IgE, IgG, and IgG4, and basophil degranulation inhibition was assessed in humanised RBL cells and blood from allergic individuals. We used DropMap microfluidics for single\u2010cell affinity profiling of IgG\u2010 and IgG4\u2010secreting B cells. IgG epitopes were mapped using overlapping peptides and CLIPS technology. Candidate AIT peptides were synthesised on solid phase via Fmoc strategy, and their allergenicity was assessed in basophil degranulation and cellular antigen stimulation assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Sera from AIT\u2010treated patients exhibited elevated IgG and IgG4 levels and enhanced inhibition of basophil degranulation. Single\u2010cell analysis indicated IgG\u2010affinity maturation with AIT. IgG\u2010epitope mapping identified four distinct, non\u2010overlapping IgG\u2010binding regions on\n                      <jats:italic>Bet v 1<\/jats:italic>\n                      . Six peptides derived from these regions were successfully produced and did not induce basophil degranulation in\u00a0vitro. The application of DropMap microfluidics allowed high\u2010resolution single B\u2010cell analysis, providing novel insights into allergen\u2010specific B\u2010cell responses and the effects of AIT at the clonal level.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This integrated approach identifies hypoallergenic peptides that selectively engage protective IgG responses and offers a framework for developing safer, next\u2010generation immunotherapies for allergic disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cea.70276","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T16:25:53Z","timestamp":1773159953000},"page":"881-892","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Patient\u2010Derived\n                    <scp>IgG<\/scp>\n                    Epitope Mapping of Bet v 1 Reveals Hypoallergenic Peptide Candidates for Safe and Next\u2010Generation Allergen Immunotherapy"],"prefix":"10.1111","volume":"56","author":[{"given":"Lara","family":"\u0160o\u0161i\u0107","sequence":"first","affiliation":[{"name":"Department of Dermatology University of Zurich  Schlieren Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5371-0514","authenticated-orcid":false,"given":"Marta","family":"Paolucci","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Zurich  Schlieren Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0025-8023","authenticated-orcid":false,"given":"Alessandro","family":"Streuli","sequence":"additional","affiliation":[{"name":"Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences ETH Zurich  Zurich Switzerland"},{"name":"Laboratory for Pharmaceutical Analytics, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences ETH Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Melillo","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Zurich  Schlieren Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianchi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaela","family":"Campana","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0469-7661","authenticated-orcid":false,"given":"Claudia C. V.","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Zurich  Schlieren Switzerland"},{"name":"Department of Dermatology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"K\u00fcndig","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Zurich  Schlieren Switzerland"},{"name":"Department of Dermatology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6102-3367","authenticated-orcid":false,"given":"Christian","family":"Steuer","sequence":"additional","affiliation":[{"name":"Laboratory for Pharmaceutical Analytics, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences ETH Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9344-5110","authenticated-orcid":false,"given":"Klaus","family":"Eyer","sequence":"additional","affiliation":[{"name":"Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences ETH Zurich  Zurich Switzerland"},{"name":"Department of Biomedicine Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5055-6299","authenticated-orcid":false,"given":"P\u00e5l","family":"Johansen","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Zurich  Schlieren Switzerland"},{"name":"Department of Dermatology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/1744666X.2023.2209319"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanepe.2021.100275"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.12.1080"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.05.039"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.05.038"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202011-4107OC"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03636-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1934"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40521-013-0006-5"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb05000.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2018.12.011"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.11.011"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.09.027"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.13840"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12835"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15007-013-0350-6"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/clt2.12245"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14073"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2023.01.005"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3964"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.2.231"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.6.3084"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.02081.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000804"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.1.331"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.10.026"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01703-2"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.2.956"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202441"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12236"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.05.025"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.10.048"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.01.016"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15865"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00233-X"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2034"},{"key":"e_1_2_10_38_1","unstructured":"E.Mathieu H.Ritchie L.Rod\u00e9s\u2010Guirao et\u00a0al. \u201cCoronavirus (COVID\u201019) Vaccinations \u201d2020."},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25894"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15311"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-020-00273-5"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.70276","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cea.70276","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cea.70276","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:16:46Z","timestamp":1785716206000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cea.70276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,10]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/cea.70276"],"URL":"https:\/\/doi.org\/10.1111\/cea.70276","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,10]]},"assertion":[{"value":"2025-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}