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In the absence of clonal mast cell disease and hereditary \u03b1\u2010tryptasemia (H\u03b1T), the contribution of the tryptase locus variation to BST and anaphylaxis remains undefined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the impact of tryptase locus variation on BST and anaphylaxis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Individuals with systemic\n                      <jats:italic>Hymenoptera<\/jats:italic>\n                      venom allergic reactions, large local reactions and asymptomatic sensitization underwent a novel ddPCR\u2010based tryptase genotyping to quantify active tryptase alleles. Individuals positive for\n                      <jats:italic>KIT<\/jats:italic>\n                      p.D816V or H\u03b1T were excluded.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The cohort consisted of 295 individuals with systemic reactions, 140 with large local reactions, and 29 asymptomatically sensitized individuals. Active tryptase allele number, excluding the inactive \u03b2IIIFS allele, correlated with BST levels (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.34,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001); all BST values were within the normal range. Patients with systemic reactions had higher BST compared with those with large local reactions and asymptomatic sensitization (median, 4.5\u2009ng\/mL vs. 3.5\u2009ng\/mL;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Individuals carrying four active \u03b2\u2010tryptase alleles were less likely to experience anaphylaxis than those carrying two or three active \u03b2\u2010tryptase alleles (26.7% vs. 42.3%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.024). A trend towards more severe reactions was observed in individuals carrying \u03b1\u2010tryptase allele together with a higher number of active \u03b2\u2010tryptase alleles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Variation at the tryptase locus explains differences in BST levels within the normal range. Four active \u03b2\u2010tryptase alleles were associated with a lower frequency of anaphylaxis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cea.70448","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:05:25Z","timestamp":1791439525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tryptase Locus Variation Contributes to Normal\u2010Range Basal Serum Tryptase Variability and Is Associated With Anaphylaxis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2562-4350","authenticated-orcid":false,"given":"Manca","family":"Svetina","sequence":"first","affiliation":[{"name":"University Clinic of Respiratory and Allergic Diseases  Golnik Slovenia"},{"name":"Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2346-8189","authenticated-orcid":false,"given":"Jonathan J.","family":"Lyons","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology, Department of Medicine University of California San Diego  La Jolla California USA"},{"name":"Veterans Affairs San Diego Healthcare System  La Jolla California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7261-6689","authenticated-orcid":false,"given":"Peter","family":"Kopa\u010d","sequence":"additional","affiliation":[{"name":"University Clinic of Respiratory and Allergic Diseases  Golnik Slovenia"},{"name":"Medical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julij","family":"\u0160elb","sequence":"additional","affiliation":[{"name":"University Clinic of Respiratory and Allergic Diseases  Golnik Slovenia"},{"name":"Medical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4701-7374","authenticated-orcid":false,"given":"Mitja","family":"Ko\u0161nik","sequence":"additional","affiliation":[{"name":"University Clinic of Respiratory and Allergic Diseases  Golnik Slovenia"},{"name":"Medical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2596-4952","authenticated-orcid":false,"given":"Matija","family":"Rijavec","sequence":"additional","affiliation":[{"name":"University Clinic of Respiratory and Allergic Diseases  Golnik Slovenia"},{"name":"Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"},{"name":"Faculty of Medicine University of Maribor  Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0835-1599","authenticated-orcid":false,"given":"Peter","family":"Koro\u0161ec","sequence":"additional","affiliation":[{"name":"University Clinic of Respiratory and Allergic Diseases  Golnik Slovenia"},{"name":"Faculty of Pharmacy University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"273","volume-title":"Basic Immunology: Functions and Disorders of the Immune System","author":"Abbas A. 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