{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:39:15Z","timestamp":1792683555745,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Experimental Allergy"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Peanut allergy remains a clinical challenge, with limited treatment options such as omalizumab (Xolair) and allergen immunotherapy (AIT). However, these are constrained by eligibility and safety concerns. AIT induces protective allergen\u2010neutralizing IgG4 in most, but not all patients. Allergen\u2010neutralizing IgG4 competes with allergen\u2010specific IgE, inhibits Fc\u03b5RI\u2010mediated mast cell activation, and inhibits IgE\u2010facilitated antigen presentation via CD23. Recent findings suggest that combining omalizumab with AIT enhances safety and reduces side effects. We hypothesize that controlled allergen exposure combined with peanut\u2010specific IgG4 mAbs (MY\u20104\u2010mAbs) prevents IgE binding to peanut allergens, reduces CD23\u2010mediated allergen presentation and Th2 responses, and skews immunity towards protective allergen\u2010specific IgG while limiting allergen\u2010specific IgE induction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>PBMCs from peanut\u2010allergic donors were exposed to Ara h 2 with or without MY\u20104\u2010mAbs. Th2 cytokines, Foxp3+ Tregs, and IgE\u2010facilitated allergen binding (FAB) to B cells were assessed. In\u00a0vivo, peanut\u2010sensitized C3H\/HeJ mice underwent oral\u2010AIT with peanut extract with or without MY\u20104\u2010mAbs and were monitored for anaphylaxis during and after oral\u2010AIT. Additionally, peanut\u2010specific IgG and IgE levels were measured before and after oral\u2010AIT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ex vivo, MY\u20104\u2010mAbs with Ara h 2 suppressed Th2 cytokines, enhanced IL\u201010 secretion, and inhibited FAB. MY\u20104\u2010mAbs redirected allergen binding to myeloid antigen\u2010presenting cells and promoted tolerance\u2010associated responses. In\u00a0vivo, the combination maintained peanut\u2010specific IgG induced during oral\u2010AIT, prevented increases in IgE, and reduced mast cell activation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Allergen\u2010specific IgG4 mAbs with controlled allergen exposure may improve the safety and efficacy of peanut AIT while promoting tolerance\u2010associated immune responses, which might allow disease modification towards allergen tolerance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cea.70459","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:47:05Z","timestamp":1792680425000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Peanut\u2010Specific\n                    <scp>IgG4 mAbs<\/scp>\n                    Promote Regulatory Immune Responses Ex\u00a0Vivo and Improve Oral Immunotherapy in Mice"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3708-1660","authenticated-orcid":false,"given":"Pascal","family":"Gasser","sequence":"first","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0274-1797","authenticated-orcid":false,"given":"Alex A. J.","family":"Overman","sequence":"additional","affiliation":[{"name":"Cell Biology and Immunology Wageningen University &amp; Research  Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6446-9148","authenticated-orcid":false,"given":"Mojtaba","family":"Porbahaie","sequence":"additional","affiliation":[{"name":"Cell Biology and Immunology Wageningen University &amp; Research  Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Arena","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane","family":"Konrad","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Repke","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"G\u00f6tschi","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitri","family":"Bieli","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Sonati","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alcide","family":"Barberis","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niccol\u00f2","family":"Pengo","sequence":"additional","affiliation":[{"name":"Mabylon AG  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3991-5297","authenticated-orcid":false,"given":"R. 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