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Further characterization of subtype is possible by identification of the target antigen by immunoblotting. However, immunoblotting is time-consuming and may not be practical for routine use in the laboratory. In this report, we describe a simple technique to identify the target antigen by indirect immunofluorescence, using epidermolysis bullosa skin as substrate.<\/jats:p>","DOI":"10.1111\/ced.12115","type":"journal-article","created":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T05:43:18Z","timestamp":1363844598000},"page":"289-294","source":"Crossref","is-referenced-by-count":6,"title":["Antigen identification using skin deficient in basement-membrane protein: a novel tool for the diagnosis of subepidermal immunobullous diseases"],"prefix":"10.1093","volume":"38","author":[{"given":"R.","family":"Rao","sequence":"first","affiliation":[{"name":"Department of Dermatology Kasturba Medical College Madhavnagar Manipal India"},{"name":"Department of Immunodermatology St. John's Institute of Dermatology St. Thomas' Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Bhogal","sequence":"additional","affiliation":[{"name":"Department of Immunodermatology St. John's Institute of Dermatology St. Thomas' Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Groves","sequence":"additional","affiliation":[{"name":"Department of Immunodermatology St. John's Institute of Dermatology St. Thomas' Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,4,1]]},"reference":[{"key":"2023041918360875600_ced12115-bib-0001","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1046\/j.1365-2133.2000.03841.x","article-title":"Autoantibodies in anti- 200 pemphigoid stain skin lacking laminin 5 and type VII collagen","volume":"143","author":"Zillikens","year":"2000","journal-title":"Br J Dermatol"},{"key":"2023041918360875600_ced12115-bib-0002","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0190-9622(00)90101-4","article-title":"A subepidermal bullous eruption associated with IgG autoantibodies to a 200 kd dermal antigen: the first case report from the United States","volume":"42","author":"Mascar\u00f3","year":"2000","journal-title":"J Am Acad Dermatol"},{"key":"2023041918360875600_ced12115-bib-0003","first-page":"399","article-title":"The diagnosis and treatment of autoimmune blistering skin diseases","volume":"108","author":"Schmidt","year":"2011","journal-title":"Dtsch Arztebl Int"},{"key":"2023041918360875600_ced12115-bib-0004","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/S0923-1811(99)00019-5","article-title":"Acquired skin disease of hemidesmosomes","volume":"20","author":"Zillikens","year":"1999","journal-title":"J Dermatol Sci"},{"key":"2023041918360875600_ced12115-bib-0005","first-page":"40.1","volume-title":"Rook's Textbook of Dermatology","author":"Wojnarowska","year":"2010","edition":"7"},{"key":"2023041918360875600_ced12115-bib-0006","first-page":"19","article-title":"Pemphigoid mimicking epidermolysis bullosa acquisita","volume":"122","author":"Delaporte","year":"1995","journal-title":"Ann Dermatol Venereol"},{"key":"2023041918360875600_ced12115-bib-0007","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/S0190-9622(84)80459-4","article-title":"Epidermolysis bullosa acquisita: a pemphigoid-like disease","volume":"11","author":"Gammon","year":"1984","journal-title":"J Am Acad Dermatol"},{"key":"2023041918360875600_ced12115-bib-0008","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1046\/j.1365-2133.1997.d01-1181.x","article-title":"Dermatitis herpetiformis and bullous pemphigoid: a developing association confirmed by immunoelectronmicroscopy","volume":"136","author":"Setterfield","year":"1997","journal-title":"Br J Dermatol"},{"key":"2023041918360875600_ced12115-bib-0009","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1046\/j.1365-2133.1999.03041.x","article-title":"Defining target antigens in linear IgA disease using skin from subjects with inherited epidermolysis bullosa as a substrate for indirect immunofluorescence microscopy","volume":"141","author":"Harman","year":"1999","journal-title":"Br J Dermatol"},{"key":"2023041918360875600_ced12115-bib-0010","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.clindermatol.2011.03.012","article-title":"Inherited epidermolysis bullosa. 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