{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:53:16Z","timestamp":1788234796613,"version":"build-2803163510"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Deputy of Research, Tehran University of Medical Sciences","award":["ID 8800"],"award-info":[{"award-number":["ID 8800"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Drug withdrawal is the ultimate goal in the management of patients with pemphigus. Direct immunofluorescence (DIF) has long been considered the gold-standard test to predict immunological remission in pemphigus vulgaris (PV); however, there have been no comparisons between DIF and antidesmoglein (anti-Dsg) ELISA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>To compare anti-Dsg ELISA with DIF in patients with PV for evaluation of immunological remission.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The study enrolled 46 patients with PV who had absence of any lesion, and had a daily prednisolone dosage of \u2264 10 mg without adjuvant drug treatment in the preceding 6 months. Biopsy specimens were taken from patients and processed for DIF. Intercellular deposition of IgG and\/or C3 was considered positive. Serum samples were also collected for anti-Dsg1 and anti-Dsg3 ELISA, and an ELISA index value of &amp;gt; 20.0 was considered positive.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>DIF and anti-Dsg ELISA were positive for 11 (23.9%) and 18 patients (39.1%), respectively. Anti-Dsg ELISA had a sensitivity of 100%, a specificity of 80%, a positive predictive value of 61.1% and a negative predictive value of 100%.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The high sensitivity of anti-Dsg ELISA proves that this simple serological test is a good substitute for DIF for evaluation of immunological remission in PV. As none of the DIF-positive patients was anti-Dsg-negative, it is possible that during the course of immunological remission, results for DIF may become negative before the results for Dsg ELISA do so.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/ced.12187","type":"journal-article","created":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T11:53:16Z","timestamp":1371642796000},"page":"41-47","source":"Crossref","is-referenced-by-count":27,"title":["Comparison of desmoglein 1 and 3 enzyme-linked immunosorbent assay and direct immunofluorescence for evaluation of immunological remission in pemphigus vulgaris"],"prefix":"10.1093","volume":"39","author":[{"given":"M.","family":"Daneshpazhooh","sequence":"first","affiliation":[{"name":"Department of Dermatology Autoimmune Bullous Diseases Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Kamyab","sequence":"additional","affiliation":[{"name":"Department of Dermatopathology Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.-S.","family":"Kalantari","sequence":"additional","affiliation":[{"name":"Department of Dermatology Autoimmune Bullous Diseases Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Balighi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Autoimmune Bullous Diseases Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. S.","family":"Naraghi","sequence":"additional","affiliation":[{"name":"Department of Dermatopathology Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Shamohammadi","sequence":"additional","affiliation":[{"name":"Medical Laboratory Razi Hospital Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.-A.","family":"Mortazavizadeh","sequence":"additional","affiliation":[{"name":"Department of Dermatology Autoimmune Bullous Diseases Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ramezani","sequence":"additional","affiliation":[{"name":"Department of Dermatology Autoimmune Bullous Diseases Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Chams-Davatchi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Autoimmune Bullous Diseases Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2023041919081030800_ced12187-bib-0001","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1111\/j.1365-2133.2003.05665.x","article-title":"Guidelines for the management of pemphigus vulgaris","volume":"149","author":"Harman","year":"2003","journal-title":"Br J Dermatol"},{"key":"2023041919081030800_ced12187-bib-0002","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.annder.2011.01.017","article-title":"[Linear IgA dermatosis. Guidelines for the diagnosis and treatment. Centres de r\u00e9f\u00e9rence des maladies bulleuses auto-immunes. 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