{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T15:09:40Z","timestamp":1774451380677,"version":"3.50.1"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Wissenschaftliches Herausgeberkollegium der M\u00fcnchener Medizinischen Wochenschrift e.V."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,4,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Indirect immunofluorescence (IIF) microscopy on monkey oesophagus is an important assay for the diagnosis of bullous pemphigoid (BP). Its relatively low sensitivity (60\u201380%) may be partly due to insufficient detection of minor IgG subclasses.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>To determine the operating characteristics of an IgG subclass in IIF.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We designed a retrospective, dual-centre, controlled cohort study on sera from 64 BP sera that had been rated as false negatives by traditional IIF microscopy, and assessed circulating IgG1, IgG3 and IgG4 autoantibodies.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The sensitivities of IIF in detecting IgG1, IgG3, IgG4 and all three in combination were 45.3%, 18.8%, 32.8% and 48.4%, respectively. Specificities were &amp;gt; 97%.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Detection of IgG subclass (especially IgG1 and IgG4) autoantibodies by IIF on monkey oesophagus can significantly improve diagnostic performance of IIF microscopy for diagnosis of BP.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/ced.13371","type":"journal-article","created":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T04:56:46Z","timestamp":1515992206000},"page":"248-253","source":"Crossref","is-referenced-by-count":13,"title":["Increased sensitivity and high specificity of indirect immunofluorescence in detecting IgG subclasses for diagnosis of bullous pemphigoid"],"prefix":"10.1093","volume":"43","author":[{"given":"J.","family":"Jank\u00e1skov\u00e1","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig Maximilian University Munich Germany"},{"name":"Department of Dermato-Venerology Teaching Hospital Kr\u00e1lovsk\u00e9 Vinohrady Third Faculty of Medicine Charles University in Prague Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. N.","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig Maximilian University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Varga","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig Maximilian University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Arenberger","sequence":"additional","affiliation":[{"name":"Department of Dermato-Venerology Teaching Hospital Kr\u00e1lovsk\u00e9 Vinohrady Third Faculty of Medicine Charles University in Prague Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Allergology and Venerology University of L\u00fcbeck L\u00fcbeck Germany"},{"name":"L\u00fcbeck Institute of Experimental Dermatology (LIED) University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Ruzicka","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig Maximilian University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"S\u00e1rdy","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig Maximilian University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,4,1]]},"reference":[{"key":"2023041918363891100_ced13371-bib-0001","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s00105-015-3649-2","article-title":"Pemphigoiderkrankungen: autoimmunerkrankungen des Alters [Pemphigoid diseases: autoimmune diseases in the 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