{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T07:20:00Z","timestamp":1772263200243,"version":"3.50.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2012,12,3]],"date-time":"2012-12-03T00:00:00Z","timestamp":1354492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,12,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>National Institute of Clinical Excellence (NICE) and European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) guidance for the diagnosis of coeliac disease has been published. However, there is some controversy regarding the advice on the use of stratifying levels of immunoglobulin (IgA) tissue transglutaminase antibody (TG2) test positivity in the absence of test standardization and the vagueness of the indication to test equivocal samples. Using repeat service audit, we demonstrate that a combination of TG2 followed by IgA endomysial antibodies (EMA) is the best strategy for all degrees of mucosal abnormality using our test combination. Reliance upon immunoassay titre is not as effective, and cannot be applied consistently across populations in the absence of assay standardization. Guidelines advocating the use of tests should involve experts in laboratory diagnostics and external quality assurance to ensure that errors of generalization do not occur and that test performance is achievable in routine diagnostic use.<\/jats:p>","DOI":"10.1111\/cei.12000","type":"journal-article","created":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T08:32:29Z","timestamp":1350289949000},"page":"100-106","source":"Crossref","is-referenced-by-count":20,"title":["Quality not quantity for transglutaminase antibody 2: the performance of an endomysial and tissue transglutaminase test in screening coeliac disease remains stable over time"],"prefix":"10.1093","volume":"171","author":[{"given":"K","family":"Swallow","sequence":"first","affiliation":[{"name":"Immunology Department and Protein Reference Unit, Northern General Hospital, Sheffield, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Wild","sequence":"additional","affiliation":[{"name":"Immunology Department and Protein Reference Unit, Northern General Hospital, Sheffield, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Sargur","sequence":"additional","affiliation":[{"name":"Immunology Department and Protein Reference Unit, Northern General Hospital, Sheffield, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D S","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Royal Hallamshire Hospital, Sheffield, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Aziz","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Royal Hallamshire Hospital, Sheffield, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A D","family":"Hopper","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Royal Hallamshire Hospital, Sheffield, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Egner","sequence":"additional","affiliation":[{"name":"Immunology Department and Protein Reference Unit, Northern General Hospital, Sheffield, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,12,3]]},"reference":[{"key":"2021122009520796400_cei12000-bib-0001","volume-title":"Coeliac disease: recognition and assessment of coeliac disease","author":"National Institute of Clinical Excellence (NICE) guideline 86","year":"2009"},{"key":"2021122009520796400_cei12000-bib-0002","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/MPG.0b013e31821a23d0","article-title":"European Society for Pediatric Gastroenterology, Hepatology, and Nutrition guidelines for the diagnosis of coeliac disease","volume":"54","author":"Husby","year":"2012","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"2021122009520796400_cei12000-bib-0003","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.cgh.2007.12.008","article-title":"What is the role of serologic testing in celiac disease? A prospective, biopsy-confirmed study with economic analysis","volume":"6","author":"Hopper","year":"2008","journal-title":"Clin Gastroenterol Hepatol"},{"key":"2021122009520796400_cei12000-bib-0004","first-page":"241","article-title":"IgA-antitissue transglutaminase: validation of a commercial assay for diagnosing coeliac disease","volume":"48","author":"West","year":"2002","journal-title":"Clin Lab"},{"key":"2021122009520796400_cei12000-bib-0005","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1136\/bmj.39133.668681.BE","article-title":"Pre-endoscopy serological testing for coeliac disease: evaluation of a clinical decision tool","volume":"334","author":"Hopper","year":"2007","journal-title":"BMJ"},{"key":"2021122009520796400_cei12000-bib-0006","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1111\/j.1365-2796.2011.02380.x","article-title":"What is the use of biopsy and antibodies in coeliac disease diagnosis?","volume":"269","author":"Evans","year":"2011","journal-title":"J Intern Med"},{"key":"2021122009520796400_cei12000-bib-0007","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.prp.2011.08.001","article-title":"Clinical value of duodenal biopsies\u2009\u2013\u2009beyond the diagnosis of coeliac disease","volume":"207","author":"Walker","year":"2011","journal-title":"Pathol Res Pract"},{"key":"2021122009520796400_cei12000-bib-0008","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1111\/j.1365-2036.2010.04477.x","article-title":"A prospective study into the aetiology of lymphocytic duodenosis","volume":"32","author":"Aziz","year":"2010","journal-title":"Aliment Pharm Ther"},{"key":"2021122009520796400_cei12000-bib-0009","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1053\/j.gastro.2010.04.007","article-title":"Detection of celiac disease and lymphocytic enteropathy by parallel serology and histopathology in a population-based study","volume":"139","author":"Walker","year":"2010","journal-title":"Gastroenterology"},{"key":"2021122009520796400_cei12000-bib-0010","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31826531f6","article-title":"ESPGHAN guidance on coeliac disease 2012: multiples of the upper limit of normal for decision making do not harmonise assay performance across centres","volume-title":"J Pediatr Gastroenterol Nutr","author":"Egner","year":"2012"},{"key":"2021122009520796400_cei12000-bib-0011","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/S0140-6736(03)14027-5","article-title":"Coeliac disease","volume":"362","author":"Green","year":"2003","journal-title":"Lancet"},{"key":"2021122009520796400_cei12000-bib-0012","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1111\/j.1572-0241.2005.00244.x","article-title":"Anti-tissue transglutaminase antibodies in patients with abnormal liver tests: is it always coeliac disease?","volume":"100","author":"Lo Iacono","year":"2005","journal-title":"Am J Gastroenterol"},{"key":"2021122009520796400_cei12000-bib-0013","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1097\/00042737-200605000-00006","article-title":"Diagnostic accuracy of coeliac serological tests: a prospective study","volume":"18","author":"Reeves","year":"2006","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"2021122009520796400_cei12000-bib-0014","doi-asserted-by":"crossref","first-page":"346","DOI":"10.7861\/clinmedicine.9-4-346","article-title":"Discrepancies between histology and serology for the diagnosis of coeliac disease in a district general hospital: is this an unrecognised problem in other hospitals?","volume":"9","author":"Sweis","year":"2009","journal-title":"Clin Med"},{"key":"2021122009520796400_cei12000-bib-0015","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1136\/bmj.39316.442338.AD","article-title":"Adult coeliac disease","volume":"335","author":"Hopper","year":"2007","journal-title":"BMJ"},{"key":"2021122009520796400_cei12000-bib-0016","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1080\/09674845.2003.11783671","article-title":"Anti-transglutaminase antibodies and the serological diagnosis of coeliac disease","volume":"60","author":"Feighery","year":"2003","journal-title":"Br J Biomed Sci"},{"key":"2021122009520796400_cei12000-bib-0017","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1111\/j.1365-2036.2009.03982.x","article-title":"Changing patterns of coeliac serology requests","volume":"29","author":"Evans","year":"2009","journal-title":"Aliment Pharmacol Ther"},{"key":"2021122009520796400_cei12000-bib-0018","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1097\/00042737-200309000-00010","article-title":"A comparison of antibodies to tissue transglutaminase with conventional serological tests in the diagnosis of coeliac disease","volume":"15","author":"Johnston","year":"2003","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"2021122009520796400_cei12000-bib-0019","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1080\/028134300750018990","article-title":"Endomysium antibodies are superior to gliadin antibodies in screening for coeliac disease in patients presenting supposed functional gastrointestinal symptoms","volume":"18","author":"Agreus","year":"2000","journal-title":"Scand J Prim Health Care"},{"key":"2021122009520796400_cei12000-bib-0020","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1097\/00042737-200405000-00005","article-title":"Is immunoglobulin A anti-tissue transglutaminase antibody a reliable serological marker of coeliac disease?","volume":"16","author":"Lock","year":"2004","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"2021122009520796400_cei12000-bib-0021","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1111\/j.1365-2036.2008.03609.x","article-title":"Coeliac disease: a biopsy is not always necessary for diagnosis","volume":"27","author":"Hill","year":"2008","journal-title":"Aliment Pharmacol Ther"},{"key":"2021122009520796400_cei12000-bib-0022","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1080\/00365520410008051","article-title":"IgA antibodies to human tissue transglutaminase: audit of routine practice confirms high diagnostic accuracy","volume":"39","author":"Hill","year":"2004","journal-title":"Scand J Gastroenterol"},{"key":"2021122009520796400_cei12000-bib-0023","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1080\/0031302031000150542","article-title":"Antibody and genetic testing in coeliac disease","volume":"35","author":"Wong","year":"2003","journal-title":"Pathology"},{"key":"2021122009520796400_cei12000-bib-0024","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1373\/clinchem.2004.035832","article-title":"Diagnostic accuracy of ten second-generation (human) tissue transglutaminase antibody assays in celiac disease","volume":"50","author":"Van Meensel","year":"2004","journal-title":"Clin Chem"},{"issue":"Pt 2","key":"2021122009520796400_cei12000-bib-0025","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1258\/000456307780117911","article-title":"A review of the quality of gastrointestinal investigations performed in UK laboratories","volume":"44","author":"Duncan","year":"2007","journal-title":"Ann Clin Biochem"},{"key":"2021122009520796400_cei12000-bib-0026","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/ajg.2008.8","article-title":"A report on the International Transglutaminase Autoantibody Workshop for Coeliac Disease","volume":"104","author":"Li","year":"2009","journal-title":"Am J Gastroenterol"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcei.12000","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/171\/1\/100\/41788598\/cei12000.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/171\/1\/100\/41788598\/cei12000.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T16:19:00Z","timestamp":1641053940000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/171\/1\/100\/6420920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,3]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,12,3]]},"published-print":{"date-parts":[[2012,12,3]]}},"URL":"https:\/\/doi.org\/10.1111\/cei.12000","relation":{},"ISSN":["1365-2249","0009-9104"],"issn-type":[{"value":"1365-2249","type":"electronic"},{"value":"0009-9104","type":"print"}],"subject":[],"published-other":{"date-parts":[[2013,1]]},"published":{"date-parts":[[2012,12,3]]}}}