{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:52:01Z","timestamp":1789390321818,"version":"build-2803163510"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2013,6,6]],"date-time":"2013-06-06T00:00:00Z","timestamp":1370476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"NIH","award":["R01 AI 047040"],"award-info":[{"award-number":["R01 AI 047040"]}]},{"name":"NIH","award":["R01 AI 054843"],"award-info":[{"award-number":["R01 AI 054843"]}]},{"name":"NIH","award":["M03 RR 30"],"award-info":[{"award-number":["M03 RR 30"]}]},{"name":"NIH","award":["T32 AI 007062 28A2"],"award-info":[{"award-number":["T32 AI 007062 28A2"]}]},{"name":"NIH","award":["2 K12 HD043494 06"],"award-info":[{"award-number":["2 K12 HD043494 06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>The development of T cells with a regulatory phenotype after thymus transplantation has not been examined previously in complete DiGeorge anomaly (cDGA). Seven athymic infants with cDGA and non-maternal pretransplantation T cell clones were assessed. Pretransplantation forkhead box protein 3 (Foxp3)+ T cells were detected in five of the subjects. Two subjects were studied in greater depth. T cell receptor variable \u03b2 chain (TCR-V\u03b2) expression was assessed by flow cytometry. In both subjects, pretransplantation FoxP3+ and total CD4+ T cells showed restricted TCR-V\u03b2 expression. The development of naive T cells and diverse CD4+ TCR-V\u03b2 repertoires following thymic transplantation indicated successful thymopoiesis from the thymic tissue grafts. Infants with atypical cDGA develop rashes and autoimmune phenomena before transplantation, requiring treatment with immunosuppression, which was discontinued successfully subsequent to the observed thymopoiesis. Post-transplantation, diverse TCR-V\u03b2 family expression was also observed in FoxP3+ CD4+ T cells. Interestingly, the percentages of each of the TCR-V\u03b2 families expressed on FoxP3+ and total CD4+ T cells differed significantly between these T lymphocyte subpopulations before transplantation. By 16 months post-transplantation, however, the percentages of expression of each TCR-V\u03b2 family became significantly similar between FoxP3+ and total CD4+ T cells. Sequencing of TCRBV DNA confirmed the presence of clonally amplified pretransplantation FoxP3+ and FoxP3\u2212 T cells. After thymus transplantation, increased polyclonality was observed for both FoxP3+ and FoxP3\u2212 cells, and pretransplantation FoxP3+ and FoxP3\u2212 clonotypes essentially disappeared. Thus, post-transplantation thymic function was associated with the development of a diverse repertoire of FoxP3+ T cells in cDGA, corresponding with immunological and clinical recovery.<\/jats:p>","DOI":"10.1111\/cei.12088","type":"journal-article","created":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T10:35:56Z","timestamp":1361356556000},"page":"140-149","source":"Crossref","is-referenced-by-count":26,"title":["Thymus transplantation restores the repertoires of forkhead box protein 3 (FoxP3)+ and FoxP3\u2212 T cells in complete DiGeorge anomaly"],"prefix":"10.1093","volume":"173","author":[{"given":"I K","family":"Chinn","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Allergy and Immunology, Duke University Medical Center, Durham, NC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J D","family":"Milner","sequence":"additional","affiliation":[{"name":"Laboratory of Allergic Diseases, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Scheinberg","sequence":"additional","affiliation":[{"name":"Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA"},{"name":"Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D C","family":"Douek","sequence":"additional","affiliation":[{"name":"Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M L","family":"Markert","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Allergy and Immunology, Duke University Medical Center, Durham, NC, USA"},{"name":"Department of Immunology, Duke University Medical Center, Durham, NC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,6,6]]},"reference":[{"key":"2021122009555373000_cei12088-bib-0001","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/S0022-3476(65)81796-6","article-title":"Discussion of Cooper MD, Peterson RDA, Good RA. 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