{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T15:56:07Z","timestamp":1769874967949,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2014,1,3]],"date-time":"2014-01-03T00:00:00Z","timestamp":1388707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Mexican Scholarship by the Mexican Council for Science and Technology"},{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"publisher","award":["406656"],"award-info":[{"award-number":["406656"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"publisher","award":["361646"],"award-info":[{"award-number":["361646"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Walter and Eliza Hall Institute and Melbourne Health"},{"DOI":"10.13039\/100010847","name":"Coeliac Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>T cell cytokine release assays are used to diagnose infectious diseases, but not autoimmune or allergic disease. Coeliac disease (CD) is a common T cell-mediated disease diagnosed by the presence of gluten-dependent intestinal inflammation and serology. Many patients cannot be diagnosed with CD because they reduce dietary gluten before medical workup. Oral gluten challenge in CD patients treated with gluten-free diet (GFD) mobilizes gluten-reactive T cells measurable by interferon (IFN)-\u03b3 enzyme-linked immunospot (ELISPOT) or major histocompatibility complex (MHC) class II tetramers. Immunodominant peptides are quite consistent in the 90% of patients who possess HLA-DQ2\u00b75. We aimed to develop whole blood assays to detect gluten-specific T cells. Blood was collected before and after gluten challenge from GFD donors confirmed to have CD (n = 27, all HLA-DQ2\u00b75+), GFD donors confirmed not to have CD (n = 6 HLA-DQ2\u00b75+, 11 HLA-DQ2\u00b75\u2212) and donors with CD not following GFD (n = 4, all HLA-DQ2\u00b75+). Plasma IFN-\u03b3 and IFN-\u03b3 inducible protein-10 (IP-10) were measured by enzyme-linked immunosorbent assay (ELISA) after whole blood incubation with peptides or gliadin, and correlated with IFN-\u03b3 ELISPOT. No T cell assay could distinguish between CD patients and controls prior to gluten challenge, but after gluten challenge the whole blood IFN-\u03b3 ELISA and the ELISPOT were both 85% sensitive and 100% specific for HLA-DQ2\u00b75+ CD patients; the whole blood IP-10 ELISA was 94% sensitive and 100% specific. We conclude that whole blood cytokine release assays are sensitive and specific for detection of gluten-reactive T cells in CD; further clinical studies addressing the utility of these tests in patients with an uncertain diagnosis of CD is warranted.<\/jats:p>","DOI":"10.1111\/cei.12232","type":"journal-article","created":{"date-parts":[[2013,11,6]],"date-time":"2013-11-06T09:42:39Z","timestamp":1383730959000},"page":"305-315","source":"Crossref","is-referenced-by-count":56,"title":["<i>Ex-vivo<\/i> whole blood secretion of interferon (IFN)-\u03b3 and IFN-\u03b3-inducible protein-10 measured by enzyme-linked immunosorbent assay are as sensitive as IFN-\u03b3 enzyme-linked immunospot for the detection of gluten-reactive T cells in human leucocyte antigen (HLA)-DQ2\u00b75+-associated coeliac disease"],"prefix":"10.1093","volume":"175","author":[{"given":"N","family":"Ontiveros","sequence":"first","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, Parkville, Vic., Australia"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, Vic., Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Tye-Din","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, Parkville, Vic., Australia"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, Vic., Australia"},{"name":"Department of Gastroenterology, The Royal Melbourne Hospital, Parkville, Vic., Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M Y","family":"Hardy","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, Parkville, Vic., Australia"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, Vic., Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R P","family":"Anderson","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, Parkville, Vic., Australia"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, Vic., Australia"},{"name":"ImmusanT, Inc., Cambridge, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,1,3]]},"reference":[{"key":"2021111804104876600_cei12232-bib-0001","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1038\/ajg.2012.219","article-title":"The prevalence of celiac disease in the United States","volume":"107","author":"Rubio-Tapia","year":"2012","journal-title":"Am J Gastroenterol"},{"key":"2021111804104876600_cei12232-bib-0002","volume-title":"Dieting monitor: eating patterns in America: NPD Group","author":"NPD Group","year":"2013"},{"key":"2021111804104876600_cei12232-bib-0003","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.cgh.2012.12.015","article-title":"Diagnosis of celiac disease in adults based on serology test 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