{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:01:27Z","timestamp":1753884087454,"version":"3.37.3"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2014,7,9]],"date-time":"2014-07-09T00:00:00Z","timestamp":1404864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003141","name":"CONACYT","doi-asserted-by":"publisher","award":["140998"],"award-info":[{"award-number":["140998"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"DGAPA","doi-asserted-by":"publisher","award":["IN214713-2"],"award-info":[{"award-number":["IN214713-2"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CONACyT M\u00e9xico doctoral scholarship","award":["261862"],"award-info":[{"award-number":["261862"]}]},{"name":"CONACyT M\u00e9xico master in Science scholarship","award":["288831"],"award-info":[{"award-number":["288831"]}]},{"DOI":"10.13039\/501100005739","name":"UNAM","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,7,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Tuberculosis is a disease caused by the Mycobacterium tuberculosis complex (MTb). In 2011, global mortality due to tuberculosis was 1\u00b74 million individuals. The only available vaccine is the attenuated M. bovis [bacillus Calmette\u2013Gu\u00e9rin (BCG)] strain, which confers variable protection against pulmonary tuberculosis. Some widely distributed non-tuberculous mycobacteria (NTM), such as M. avium and M. arupense, are also potential pathogens for humans. This work aimed to produce and characterize monoclonal antibodies against the M. bovis\u2005BCG Mexico strain of the MTb, M. avium subs. hominissuis and the M. arupense strain from NTM. Hybridomas were produced from splenocytes of BALB\/c female mice immunized with radiation-inactivated mycobacteria, and the immunoglobulin (Ig)G2a antibody-producing clones with the highest antigenic recognition were selected. The selected clones, Mbv 2A10 for M. bovis\u2005BCG Mexico, Mav 3H1 for M. avium and Mar 2D10 for M. arupense, were used in further studies. Enzyme-linked immunosorbent assay (ELISA) and immune proteomics analyses characterized the clones as having the highest cross-reactivity with mycobacteria. Using mass spectrometry, a number of proteins recognized by the monoclonal antibody (mAb) clones were identified. These proteins had roles in metabolic processes, hypoxia, cell cycle and dormancy. In addition, a Clustal W and Immune Epitope Database (IEDB) in-silico analysis was performed in protein sequences that result in the conserved regions within probability epitopes that could be recognized for Mbv2A10 and Mav3H1 clones.<\/jats:p>","DOI":"10.1111\/cei.12309","type":"journal-article","created":{"date-parts":[[2014,2,28]],"date-time":"2014-02-28T13:07:42Z","timestamp":1393592862000},"page":"454-463","source":"Crossref","is-referenced-by-count":4,"title":["Potential cross-reactivity of monoclonal antibodies against clinically relevant mycobacteria"],"prefix":"10.1093","volume":"177","author":[{"given":"K","family":"Flores-Moreno","sequence":"first","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J S","family":"Celis-Meneses","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D M","family":"Meneses-Ruiz","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A I","family":"Castillo-Rodal","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Ordu\u00f1a","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B A","family":"Montiel","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"L\u00f3pez-Vidal","sequence":"additional","affiliation":[{"name":"Programa de Inmunolog\u00eda Molecular Microbiana, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,7,9]]},"reference":[{"volume-title":"Global Tuberculosis Report","year":"2012","author":"World Health Organization","key":"2021122010070635500_cei12309-bib-0001"},{"key":"2021122010070635500_cei12309-bib-0002","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ccm.2005.02.004","article-title":"The origin and evolution of Mycobacterium tuberculosis","volume":"26","author":"Mostowy","year":"2005","journal-title":"Clin Chest Med"},{"volume-title":"The immunological basis for immunization series, module 5. Tuberculosis","year":"1993","author":"Milstien","key":"2021122010070635500_cei12309-bib-0003"},{"key":"2021122010070635500_cei12309-bib-0004","doi-asserted-by":"crossref","first-page":"70","DOI":"10.4161\/hv.5.2.7210","article-title":"BCG vaccines: their mechanisms of attenuation and impact on safety and protective efficacy","volume":"5","author":"Liu","year":"2009","journal-title":"Hum Vaccin"},{"key":"2021122010070635500_cei12309-bib-0005","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1186\/1471-2164-12-493","article-title":"Genomic and proteomic analyses of Mycobacterium bovis BCG Mexico 1931 reveal a diverse immunogenic repertoire against tuberculosis infection","volume":"12","author":"Ordu\u00f1a","year":"2011","journal-title":"BMC Genomics"},{"key":"2021122010070635500_cei12309-bib-0006","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.tripleo.2004.02.006","article-title":"Isolation of non-tuberculosis mycobacteria in treated dental unit waterlines","volume":"98","author":"Porteous","year":"2004","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"2021122010070635500_cei12309-bib-0007","first-page":"290","article-title":"Infections due to non-tuberculous mycobacteria (NTM)","volume":"120","author":"Katoch","year":"2004","journal-title":"Indian J Med Res"},{"key":"2021122010070635500_cei12309-bib-0008","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1183\/09031936.04.00114004","article-title":"Nontuberculous mycobacteria in non-HIV patients: epidemiology, treatment and response","volume":"23","author":"Henry","year":"2004","journal-title":"Eur Respir J"},{"key":"2021122010070635500_cei12309-bib-0009","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1136\/thx.2006.070797","article-title":"Isolation prevalence of pulmonary non-tuberculous mycobacteria in Ontario, 1997\u20132003","volume":"62","author":"Marras","year":"2007","journal-title":"Thorax"},{"key":"2021122010070635500_cei12309-bib-0010","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1007\/s10096-011-1359-y","article-title":"Potentially pathogenic nontuberculous mycobacteria found in aquatic systems. 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