{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T18:12:59Z","timestamp":1778004779244,"version":"3.51.4"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T00:00:00Z","timestamp":1402272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"publisher","award":["5K08HL091058"],"award-info":[{"award-number":["5K08HL091058"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal regulatory T cell (Treg) response and increases in T helper type 1 (Th1) and Th17 cell responses. It is unclear if dysregulation of microRNAs (miRNA) within Treg cells contributes to the abnormal inflammatory response in COPD. In this study, we aimed to compare the miRNA profile of COPD Treg cells with that of healthy controls and to explore the function of differentially expressed miRNAs. We first obtained Treg and T effector cells (Teff) from peripheral blood of non-smokers, unaffected current smokers and COPD current smokers. Then, we assessed their miRNA expression by microarray analysis followed by real-time reverse transcription\u2013polymerase chain reaction (RT\u2013PCR) validation of particular miRNAs. Six and 96 miRNAs were expressed differentially in COPD Treg cells versus\u2005Treg cells of healthy non-smokers and healthy smokers, whereas no differences were found in miRNA expression in Teff cells. We found that miR-199a-5p was repressed by approximately fourfold in Treg cells of COPD patients compared to healthy smokers (P &amp;lt; 0\u00b705). In addition, miR-199a-5p was over-expressed in Treg cells compared to Teff cells (P &amp;lt; 0\u00b7001) and had significant over-representation of its target genes in the Treg transcriptome, being associated with the transforming growth factor (TGF)-\u03b2 activation pathway (P &amp;lt; 0\u00b701). We also confirmed the function of miR-199a5p in an in-vitro loss-of-function cell model running TaqMan\u00ae arrays of the human TGF-\u03b2 pathway. These findings suggest that the abnormal repression of miR-199a-5p in patients with COPD compared to unaffected smokers may be involved in modulating the adaptive immune balance in favour of a Th1 and Th17 response.<\/jats:p>","DOI":"10.1111\/cei.12325","type":"journal-article","created":{"date-parts":[[2014,3,18]],"date-time":"2014-03-18T08:50:43Z","timestamp":1395132643000},"page":"341-352","source":"Crossref","is-referenced-by-count":34,"title":["Blunted expression of miR-199a-5p in regulatory T cells of patients with chronic obstructive pulmonary disease compared to unaffected smokers"],"prefix":"10.1093","volume":"177","author":[{"given":"W M","family":"Chatila","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Temple University School of Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G J","family":"Criner","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Temple University School of Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W W","family":"Hancock","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Akimova","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Moldover","sequence":"additional","affiliation":[{"name":"B-Tech Consulting, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J-K","family":"Chang","sequence":"additional","affiliation":[{"name":"Center for Inflammation, Translational and Clinical Lung Research, Temple University School of Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Cornwell","sequence":"additional","affiliation":[{"name":"Center for Inflammation, Translational and Clinical Lung Research, Temple University School of Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Santerre","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Diseases, Temple University School of Medicine, Philadelphia, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T J","family":"Rogers","sequence":"additional","affiliation":[{"name":"Center for Inflammation, Translational and Clinical Lung Research, Temple University School of Medicine, Philadelphia, PA, 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