{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:57:55Z","timestamp":1788188275880,"version":"build-2803163510"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Peribosch Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,10,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Somatic hypermutation (SHM) is an important step in antigen-driven B cell development creating B lymphocytes expressing high-affinity antibody receptors. It is known that the peripheral B lymphocyte compartments of healthy children and adults differ considerably. However, the development of SHM with age has not been studied in detail previously. Therefore, we used the immunoglobulin (Ig)\u03ba-restriction enzyme hot-spot mutation assay (Ig\u03ba-REHMA) to gain an estimation of SHM levels in different age groups in order to relate this to the size of the memory B lymphocyte subpopulations. We show that the level of SHM increases rapidly during the first 2 years of life. This reflects the changes of the memory B cell subpopulations, but also changes in the SHM within memory B cell subsets, probably reflecting an increase of secondary memory B cell responses.<\/jats:p>","DOI":"10.1111\/cei.12419","type":"journal-article","created":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T11:50:19Z","timestamp":1405511419000},"page":"394-398","source":"Crossref","is-referenced-by-count":19,"title":["Levels of somatic hypermutations in B cell receptors increase during childhood"],"prefix":"10.1093","volume":"178","author":[{"given":"E J H","family":"Schatorj\u00e9","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Jeroen Bosch Hospital, 's-Hertogenbosch, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G J","family":"Driessen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus MC, University Medical Centre, Rotterdam, the Netherlands"},{"name":"Department of Pediatrics, Erasmus MC, University Medical Centre, Rotterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R W N M","family":"van Hout","sequence":"additional","affiliation":[{"name":"Department of Linguistics, Radboud University, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"van der Burg","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus MC, University Medical Centre, Rotterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4311-3550","authenticated-orcid":false,"given":"E","family":"de Vries","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Jeroen Bosch Hospital, 's-Hertogenbosch, the 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