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Th17 cells have been implicated in the pathogenesis of rheumatoid arthritis (RA) and spondyloarthritides (SpA). Here, we compared the expression of CD146 on CD4+ T cells between healthy donors (HD) and patients with RA and SpA [ankylosing spondylitis (AS) or psoriatic arthritis (PsA)] and examined correlations with surface markers and cytokine secretion. Peripheral blood mononuclear cells (PBMC) were obtained from patients and controls, and synovial fluid mononuclear cells (SFMC) from patients. Cytokine production [elicited by phorbol myristate acetate (PMA)\/ionomycin] and surface phenotypes were evaluated by flow cytometry. CD146+ CD4+ and interleukin (IL)-17+ CD4+ T cell frequencies were increased in PBMC of PsA patients, compared with HD, and in SFMC compared with PBMC. CD146+ CD4+ T cells were enriched for secretion of IL-17 [alone or with IL-22 or interferon (IFN)-\u03b3] and for some putative Th17-associated surface markers (CD161 and CCR6), but not others (CD26 and IL-23 receptor). CD4+ T cells producing IL-22 or IFN-\u03b3 without IL-17 were also present in the CD146+ subset, although their enrichment was less marked. Moreover, a majority of cells secreting these cytokines lacked CD146. Thus, CD146 is not a sensitive or specific marker of Th17 cells, but rather correlates with heterogeneous cytokine secretion by subsets of CD4+ helper T cells.<\/jats:p>","DOI":"10.1111\/cei.12434","type":"journal-article","created":{"date-parts":[[2014,8,12]],"date-time":"2014-08-12T06:28:57Z","timestamp":1407824937000},"page":"378-391","source":"Crossref","is-referenced-by-count":20,"title":["Relationship of CD146 expression to secretion of interleukin (IL)-17, IL-22 and interferon-\u03b3 by CD4+ T cells in patients with inflammatory arthritis"],"prefix":"10.1093","volume":"179","author":[{"given":"C","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Cambridge, Cambridge, UK"},{"name":"Department of Rheumatology and Immunology, The First Affiliated Hospital of China Medical University, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J C","family":"Goodall","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Busch","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cambridge, Cambridge, UK"},{"name":"Department of Life Sciences, University of Roehampton, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J S H","family":"Gaston","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,2,16]]},"reference":[{"key":"2021122010102882400_cei12434-bib-0001","doi-asserted-by":"crossref","first-page":"2211","DOI":"10.1002\/eji.201242872","article-title":"Emerging new roles of Th17 cells","volume":"42","author":"Kuchroo","year":"2012","journal-title":"Eur J 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