{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:07:10Z","timestamp":1789913230921,"version":"4.0.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2015,4,23]],"date-time":"2015-04-23T00:00:00Z","timestamp":1429747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital, Tampere","award":["9P034"],"award-info":[{"award-number":["9P034"]}]},{"name":"Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital, Tampere","award":["9R040"],"award-info":[{"award-number":["9R040"]}]},{"DOI":"10.13039\/501100006706","name":"Tampere Tuberculosis Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006706","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tampere Rheumatism Association"},{"name":"Medical Research Council of the Academy of Finland"},{"name":"Finnish Cancer Foundation"},{"name":"Maire Lisko Foundation and the Sigrid Juselius Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,6,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>The expression and phosphorylation of signal transducer and activator of transcription-1 (STAT-1) have been shown to be markedly increased in the salivary gland epithelial cells of patients with primary Sj\u00f6gren's syndrome (pSS). The present aim was to investigate the activation status of different STAT proteins in peripheral blood (PB) lymphocytes and monocytes, and their correlations with clinical parameters in patients with pSS. To this end, PB samples were drawn from 16 patients with active pSS and 16 healthy blood donors, and the phosphorylation of STAT-1, -3, -4, -5 and -6 proteins was studied in T cells, B cells and monocytes using multi-colour flow cytometry. In addition, mRNA expression of STAT molecules in PB mononuclear cells (PBMC) was studied with quantitative reverse transcriptase\u2013polymerase chain reaction (RT\u2013PCR). Basal phosphorylation of STAT-5 was found to be significantly higher in PB T cells, B cells and monocytes in patients with pSS than in healthy controls. The expression of STAT-5 mRNA was not increased in PBMC. pSTAT-5 levels in B cells and monocytes showed a significant correlation with serum immunoglobulin (Ig)G levels and anti-SSB antibody titres. Constitutive STAT-5 activation in monocytes and CD4+ T cells was associated with purpura. There were no major differences in the activation of other STATs between pSS patients and healthy controls. In conclusion, STAT-5 is activated constitutively in PB leucocytes in patients with pSS, and basal STAT-5 phosphorylation seems to associate with hypergammaglobulinaemia, anti-SSB antibody production and purpura.<\/jats:p>","DOI":"10.1111\/cei.12614","type":"journal-article","created":{"date-parts":[[2015,3,2]],"date-time":"2015-03-02T22:05:13Z","timestamp":1425333913000},"page":"29-38","source":"Crossref","is-referenced-by-count":24,"title":["STAT-5 is activated constitutively in T cells, B cells and monocytes from patients with primary Sj\u00f6gren's syndrome"],"prefix":"10.1093","volume":"181","author":[{"given":"M","family":"Pertovaara","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Centre for Rheumatic Diseases, Tampere University Hospital"},{"name":"Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Silvennoinen","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Tampere, Tampere"},{"name":"Department of Internal Medicine, Tampere University Hospital, Tampere, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Isom\u00e4ki","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Centre for Rheumatic Diseases, Tampere University Hospital"},{"name":"School of Medicine, University of Tampere, Tampere"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,4,23]]},"reference":[{"key":"2021122010161377500_cei12614-bib-0001","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1097\/BOR.0b013e32833c36c5","article-title":"Pathogenesis of Sj\u00f6gren's syndrome and therapeutic consequences","volume":"22","author":"Mariette","year":"2010","journal-title":"Curr Opin Rheumatol"},{"key":"2021122010161377500_cei12614-bib-0002","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1146\/annurev-pathol-012513-104728","article-title":"Sj\u00f6gren's syndrome","volume":"9","author":"Mavragani","year":"2014","journal-title":"Annu Rev Pathol"},{"key":"2021122010161377500_cei12614-bib-0003","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1001\/archinte.165.20.2337","article-title":"The risk of lymphoma development in autoimmune diseases: a meta-analysis","volume":"165","author":"Zintzaras","year":"2005","journal-title":"Arch Intern Med"},{"key":"2021122010161377500_cei12614-bib-0004","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1136\/ard.60.5.467","article-title":"A longitudinal cohort study of Finnish patients with primary Sj\u00f6gren's syndrome. 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