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Ribosomal RNA of Propionibacterium acnes and P. granulosum was measured by real-time quantitative reverse transcription\u2013polymerase chain reaction (RT\u2013PCR) using formalin-fixed and paraffin-embedded tissue of lymph node biopsy from 65 Chinese patients with sarcoidosis, 45 with tuberculosis and 50 controls with other diseases (23 with non-specific lymphadenitis and 27 with mediastinal lymph node metastasis from lung cancer). The receiver operating characteristic (ROC) curve was analysed to determine an optimal cut-off value for diagnosis, and the diagnostic accuracy of the cut-off value was evaluated in additional tissue samples [24 patients with sarcoidosis and 22 with tuberculosis (TB)]. P. acnes or P. granulosum rRNA was detected in 48 of the 65 sarcoidosis samples but only in four of the 45 TB samples and three of the 50 control samples. Analysis of the ROC curve revealed that an optimal cut-off value of the copy number of propionibacterial rRNA for diagnosis of sarcoidosis was 50\u00b75 copies\/ml with a sensitivity and specificity of 73\u00b78 and 92\u00b76%, respectively. Based on the cut-off value, 19 of the 24 additional sarcoidosis samples exhibited positive P. acnes or P. granulosum, whereas only one of the 22 additional TB samples was positive, resulting in a sensitivity and specificity of 79\u00b72 and 95\u00b75%, respectively. These findings suggest that propionibacteria might be associated with sarcoidosis granulomatous inflammation. Detection of propionibacterial rRNA by RT\u2013PCR might possibly distinguish sarcoidosis from TB.<\/jats:p>","DOI":"10.1111\/cei.12650","type":"journal-article","created":{"date-parts":[[2015,5,11]],"date-time":"2015-05-11T03:05:03Z","timestamp":1431313503000},"page":"511-517","source":"Crossref","is-referenced-by-count":25,"title":["Real-time quantitative reverse transcription\u2013polymerase chain reaction to detect propionibacterial ribosomal RNA in the lymph nodes of Chinese patients with sarcoidosis"],"prefix":"10.1093","volume":"181","author":[{"given":"Y","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y-R","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S-S","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R P","family":"Baughman","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cincinnati Medical Center, Cincinnati, OH, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H-P","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,7,1]]},"reference":[{"key":"2021122010154965800_cei12650-bib-0001","first-page":"736","article-title":"Statement on Sarcoidosis. 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