{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:26:17Z","timestamp":1790331977290,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":2009,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004710","name":"Fondazione Umberto Veronesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004710","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programma Ricerca Indipendente \u2013 Regione Lombardia","award":["DK07698"],"award-info":[{"award-number":["DK07698"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,6,15]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>The diagnosis of primary sclerosing cholangitis (PSC) is difficult due to the lack of sensitive and specific biomarkers, as is the early diagnosis of cholangiocarcinoma (CC), a complication of PSC. The aim of this study was to identify specific serum miRNAs as diagnostic biomarkers for PSC and CC. The levels of 667 miRNAs were evaluated in 90 human serum samples (30 PSC, 30 CC and 30 control subjects) to identify disease-associated candidate miRNAs (discovery phase). The deregulated miRNAs were validated in an independent cohort of 140 samples [40 PSC, 40 CC, 20 primary biliary cirrhosis (PBC) and 40 controls]. Receiver operating characteristic (ROC) curves were established and only miRNAs with an area under the curve (AUC)\u2009&amp;gt;\u20090\u00b770 were considered useful as biomarkers. In the discovery phase we identified the following: 21 miRNAs expressed differentially in PSC, 33 in CC and 26 in both in comparison to control subjects as well as 24 miRNAs expressed differentially between PSC and CC. After the validation phase, miR-200c was found to be expressed differentially in PSC versus controls, whereas miR-483-5p and miR-194 showed deregulated expression in CC compared with controls. We also demonstrate a difference in the expression of miR-222 and miR-483-5p in CC versus PSC. Combination of these specific miRNAs further improved the specificity and accuracy of diagnosis. This study provides a basis for the use of miRNAs as biomarkers for the diagnosis of PSC and CC.<\/jats:p>","DOI":"10.1111\/cei.12776","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T06:13:30Z","timestamp":1455171210000},"page":"61-71","source":"Crossref","is-referenced-by-count":72,"title":["Serum microRNAs as novel biomarkers for primary sclerosing cholangitis and cholangiocarcinoma"],"prefix":"10.1093","volume":"185","author":[{"given":"F","family":"Bernuzzi","sequence":"first","affiliation":[{"name":"Humanitas Clinical and Research Center, Rozzano, MI, Italy"},{"name":"International Center for Digestive Health, University of Milan-Bicocca, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Marabita","sequence":"additional","affiliation":[{"name":"Unit of Computational Medicine, Karolinska Institute, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Lleo","sequence":"additional","affiliation":[{"name":"Humanitas Clinical and Research Center, Rozzano, MI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Carbone","sequence":"additional","affiliation":[{"name":"Humanitas Clinical and Research Center, Rozzano, MI, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Mirolo","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnologies and Translational Medicine, University of Milan, Humanitas Clinical and Research Center, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Marzioni","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Universit\u00e0 Politecnica Delle Marche, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Alpini","sequence":"additional","affiliation":[{"name":"Research, Central Texas Veterans Health Care System, Scott and White Digestive Disease Research Center, Scott and White, Department of Medicine, Division of Gastroenterology, Texas A&M University Health Science Center, Temple, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Alvaro","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Sapienza University of Rome, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K M","family":"Boberg","sequence":"additional","affiliation":[{"name":"Medical Department, Rikshospitalet, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Locati","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnologies and Translational Medicine, University of Milan, Humanitas Clinical and Research Center, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Torzilli","sequence":"additional","affiliation":[{"name":"Liver Surgery Unit, University of Milan School of Medicine, Cancer Center, Humanitas Clinical and Research Center, Rozzano, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Rimassa","sequence":"additional","affiliation":[{"name":"Medical Oncology and Hematology Unit, Cancer Center, Humanitas Clinical and Research Center, Rozzano, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Piscaglia","sequence":"additional","affiliation":[{"name":"Internal Medicine, Alma Mater Studiorum, University of Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X-S","family":"He","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Allergy, and Clinical Immunology, University of California at Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C L","family":"Bowlus","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, University of California Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G-X","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Allergy, and Clinical Immunology, University of California at Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M E","family":"Gershwin","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Allergy, and Clinical Immunology, University of California at Davis, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Invernizzi","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Allergy, and Clinical Immunology, University of California at Davis, Davis, CA, 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