{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T13:29:26Z","timestamp":1793194166025,"version":"4.5.0"},"reference-count":105,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":1933,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia UdeA","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,19]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Regulatory T cells (Tregs) constitute a fascinating subpopulation of CD4+ T cells due to their ability to limit the immune response against self and non-self antigens. Murine models and antibodies directed against surface and intracellular molecules have allowed elucidation of the mechanisms that govern their development and function. However, these markers used to their classification lack of specificity, as they can be expressed by activated T cells. Similarly, there are slight differences between animal models, in steady state and pathological conditions, anatomical localization and strategy of analysis by flow cytometry. Here, we revised the most common markers utilized for Treg typification by flow cytometry such as CD25, forkhead box protein 3 (FoxP3) and CD127, along with our data obtained in different body compartments of humans, mice and rats. Furthermore, we revised and determined the expression of other molecules important for the phenotypical characterization of Treg cells. We draw attention to the drawbacks of those markers used in chronic states of inflammation. However, until a specific marker for the identification of Tregs is discovered, the best combination of markers will depend upon the tissue or the degree of inflammation from which Tregs derive.<\/jats:p>","DOI":"10.1111\/cei.12804","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T09:19:30Z","timestamp":1462007970000},"page":"281-291","source":"Crossref","is-referenced-by-count":164,"title":["Phenotypical characterization of regulatory T cells in humans and rodents"],"prefix":"10.1093","volume":"185","author":[{"given":"A L","family":"Rodr\u00edguez-Perea","sequence":"first","affiliation":[{"name":"Grupo Inmunovirolog\u00eda, Facultad de Medicina, Universidad de Antioquia UdeA, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E D","family":"Arcia","sequence":"additional","affiliation":[{"name":"Grupo Inmunovirolog\u00eda, Facultad de Medicina, Universidad de Antioquia UdeA, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C M","family":"Rueda","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0885-1320","authenticated-orcid":false,"given":"P A","family":"Velilla","sequence":"additional","affiliation":[{"name":"Grupo Inmunovirolog\u00eda, Facultad de Medicina, Universidad de Antioquia UdeA, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,8,1]]},"reference":[{"key":"2021111804092983300_cei12804-bib-0001","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.4049\/jimmunol.155.3.1151","article-title":"Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases","volume":"155","author":"Sakaguchi","year":"1995","journal-title":"J Immunol"},{"key":"2021111804092983300_cei12804-bib-0002","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/ni.2554","article-title":"Regulatory T cells: recommendations to simplify the nomenclature","volume":"14","author":"Abbas","year":"2013","journal-title":"Nat Immunol"},{"key":"2021111804092983300_cei12804-bib-0003","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.cell.2008.05.009","article-title":"Regulatory T cells and immune tolerance","volume":"133","author":"Sakaguchi","year":"2008","journal-title":"Cell"},{"key":"2021111804092983300_cei12804-bib-0004","doi-asserted-by":"crossref","first-page":"e0141161","DOI":"10.1371\/journal.pone.0141161","article-title":"Differences in expression level of helios and neuropilin-1 do not distinguish thymus-derived from extrathymically-induced CD4+Foxp3+ 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