{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T18:27:22Z","timestamp":1669314442385},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":1937,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"University of Luebeck","award":["#E42-2012"],"award-info":[{"award-number":["#E42-2012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,19]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Recent data suggest a possible relationship between cystic fibrosis (CF) pharmacotherapy, Aspergillus fumigatus colonization (AC) and\/or allergic bronchopulmonary aspergillosis (ABPA). The aim of this study was to determine if anti-fungal defence mechanisms are influenced by CF pharmacotherapy, i.e. if (1) neutrophils form CF and non-CF donors differ in their ability to produce chitotriosidase (CHIT-1); (2) if incubation of isolated neutrophils with azithromycin, salbutamol, prednisolone or rhDNase might influence the CHIT-1 activity; and (3) if NETosis and neutrophil killing efficiency is influenced by rhDNase. Neutrophils were isolated from the blood of CF patients (n = 19; mean age 26\u00b78 years or healthy, non-CF donors (n = 20; 38\u00b77 years) and stimulated with phorbol-12-myristate-13-acetate (PMA), azithromycin, salbutamol, prednisolone or rhDNase. CHIT-1 enzyme activity was measured with a fluorescent substrate. NETosis was induced by PMA and neutrophil killing efficiency was assessed by a hyphae recovery assay. Neutrophil CHIT-1 activity was comparable in the presence or absence of PMA stimulation in both CF and non-CF donors. PMA stimulation and preincubation with rhDNase increased CHIT-1 activity in culture supernatants from non-CF and CF donors. However, this increase was significant in non-CF donors but not in CF patients (P &amp;lt; 0\u00b705). RhDNase reduced the number of NETs in PMA-stimulated neutrophils and decreased the killing efficiency of leucocytes in our in-vitro model. Azithromycin, salbutamol or prednisolone had no effect on CHIT-1 activity. Stimulation of isolated leucocytes with PMA and treatment with rhDNase interfered with anti-fungal defence mechanisms. However, the impact of our findings for treatment in CF patients needs to be proved in a clinical cohort.<\/jats:p>","DOI":"10.1111\/cei.12827","type":"journal-article","created":{"date-parts":[[2016,6,21]],"date-time":"2016-06-21T00:16:53Z","timestamp":1466468213000},"page":"382-391","source":"Crossref","is-referenced-by-count":1,"title":["Treatment with rhDNase in patients with cystic fibrosis alters <i>in-vitro<\/i> CHIT-1 activity of isolated leucocytes"],"prefix":"10.1093","volume":"185","author":[{"given":"M","family":"Weckmann","sequence":"first","affiliation":[{"name":"Department of Pediatric Allergy and Pulmonology, Clinic of Pediatrics UKSH, University of Luebeck, Luebeck, Germany"},{"name":"Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Schultheiss","sequence":"additional","affiliation":[{"name":"Department of Pediatric Allergy and Pulmonology, Clinic of Pediatrics UKSH, University of Luebeck, Luebeck, Germany"},{"name":"Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Hollaender","sequence":"additional","affiliation":[{"name":"Department of Pediatric Allergy and Pulmonology, Clinic of Pediatrics UKSH, University of Luebeck, Luebeck, Germany"},{"name":"Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Bobis","sequence":"additional","affiliation":[{"name":"Clinic of Internal Medicine, UKSH, University of Kiel, Kiel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Rupp","sequence":"additional","affiliation":[{"name":"Department of Molecular and Clinical Infectious Diseases, University of Luebeck, Luebeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M V","family":"Kopp","sequence":"additional","affiliation":[{"name":"Department of Pediatric Allergy and Pulmonology, Clinic of Pediatrics UKSH, University of Luebeck, Luebeck, Germany"},{"name":"Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,7,28]]},"reference":[{"key":"2021111804093186000_cei12827-bib-0001","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s10661-009-1128-x","article-title":"A study on Aspergillus species in houses of asthmatic patients from Sari City, Iran and a brief review of the health effects of exposure to indoor Aspergillus","volume":"168","author":"Hedayati","year":"2010","journal-title":"Environ Monit Assess"},{"issue":"Suppl. 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