{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T02:26:04Z","timestamp":1785637564574,"version":"3.56.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":1874,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"IRCCS Burlo Garofolo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,11,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>New data suggest the involvement of rotavirus (RV) in triggering autoimmunity in coeliac disease (CD) by molecular mimicry between the human-transglutaminase protein and the dodecapeptide (260-271 aa) of the RV protein VP7 (pVP7). To assess the role of RV in the onset of CD, we measured anti-pVP7 antibodies in the sera of children with CD and of control groups. We analysed serum samples of 118 biopsy-proven CD patients and 46 patients with potential CD; 32 children with other gastrointestinal diseases; 107 no-CD children and 107 blood donors. Using enzyme-linked immunosorbent assay (ELISA) assay, we measured immunoglobulin (Ig)A\u2013IgG antibodies against the synthetic peptides pVP7, the human transglutaminase-derived peptide (476\u2013487 aa) which shows a homology with VP7 protein and a control peptide. The triple-layered RV particles (TLPs) containing the VP7 protein and the double-layered RV-particles (DLPs) lacking the VP7 protein were also used as antigens in ELISA assay. Antibody reactivity to the RV-TLPs was positive in 22 of 118 (18%) CD patients and in both paediatric (17 of 107, 16%) and adult (29 of 107, 27%) control groups, without showing a statistically significant difference among them (P\u2009=\u20090\u00b76, P\u2009=\u20090\u00b71). Biopsy-proven CD patients as well as the adult control group demonstrated a high positive antibody reactivity against both pVP7 (34 of 118, 29% CD patients; 66 of 107, 62% adult controls) and control synthetic peptides (35 of 118, 30% CD patients; 56 of 107, 52% adult controls), suggesting a non-specific response against RV pVP7. We show that children with CD do not have higher immune reactivity to RV, thus questioning the molecular mimicry mechanism as a triggering factor of CD.<\/jats:p>","DOI":"10.1111\/cei.12855","type":"journal-article","created":{"date-parts":[[2016,8,22]],"date-time":"2016-08-22T13:37:27Z","timestamp":1471873047000},"page":"356-363","source":"Crossref","is-referenced-by-count":18,"title":["Lack of evidence of rotavirus-dependent molecular mimicry as a trigger of coeliac disease"],"prefix":"10.1093","volume":"186","author":[{"given":"F","family":"Ziberna","sequence":"first","affiliation":[{"name":"Institute of Maternal and Child Health, I.R.C.C.S. \u2018Burlo Garofolo\u2019, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"De Lorenzo","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology ICGEB, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Schiavon","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Trieste, University of Trieste, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Arnoldi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Quaglia","sequence":"additional","affiliation":[{"name":"Institute of Maternal and Child Health, I.R.C.C.S. \u2018Burlo Garofolo\u2019, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"De Leo","sequence":"additional","affiliation":[{"name":"Institute of Maternal and Child Health, I.R.C.C.S. \u2018Burlo Garofolo\u2019, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Vatta","sequence":"additional","affiliation":[{"name":"Institute of Maternal and Child Health, I.R.C.C.S. \u2018Burlo Garofolo\u2019, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Martelossi","sequence":"additional","affiliation":[{"name":"Institute of Maternal and Child Health, I.R.C.C.S. \u2018Burlo Garofolo\u2019, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O R","family":"Burrone","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology ICGEB, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Ventura","sequence":"additional","affiliation":[{"name":"Institute of Maternal and Child Health, I.R.C.C.S. \u2018Burlo Garofolo\u2019, Italy"},{"name":"Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Not","sequence":"additional","affiliation":[{"name":"Institute of Maternal and Child Health, I.R.C.C.S. \u2018Burlo Garofolo\u2019, Italy"},{"name":"Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,9,29]]},"reference":[{"key":"2021120807481069000_cei12855-bib-0001","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1097\/MPG.0b013e31804ca0ec","article-title":"Rotavirus and celiac disease: clues to the pathogenesis and perspectives on prevention","volume":"44","author":"Troncone","year":"2007","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"2021120807481069000_cei12855-bib-0002","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1172\/JCI200114235","article-title":"Mechanisms for the induction of autoimmunity by infectious 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