{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:34:36Z","timestamp":1762252476033,"version":"3.37.3"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T00:00:00Z","timestamp":1486425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000066","name":"NIEHS","doi-asserted-by":"publisher","award":["TW00621-006"],"award-info":[{"award-number":["TW00621-006"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Health of the Slovak Republic","award":["2005\/40-SZU-18"],"award-info":[{"award-number":["2005\/40-SZU-18"]}]},{"name":"FP5 EU project PLUTOCRACY","award":["QLK4-CT-2000-00279"],"award-info":[{"award-number":["QLK4-CT-2000-00279"]}]},{"name":"ITMS","award":["26240120033"],"award-info":[{"award-number":["26240120033"]}]},{"name":"Slovak Research and Development","award":["APVT-21-016504"],"award-info":[{"award-number":["APVT-21-016504"]}]},{"name":"European Union Integrated Project NewGeneris, 6th Framework Programme","award":["FOODCT-2005-016320"],"award-info":[{"award-number":["FOODCT-2005-016320"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This cross-sectional study was designed to investigate the extent of genetic susceptibility by targeting variants in interleukin (IL)\u22124\/IL-13 signalling pathways leading to atopic disease in early childhood. We evaluated involvement of five single nucleotide polymorphisms IL4 C-590T, IL13 C-1055T, IL13 Arg130Gln, IL4RA Ile50Val and IL4RA Gln576Arg, in the control of serum total and antigen-specific immunoglobulin (Ig)E levels. Furthermore, we analysed their association with changes in gene expression of five cytokines having key roles in inflammatory and anti-inflammatory immune response [IL-4, IL-13, interferon (IFN)-\u03b3, IL-8 and IL-10]. Total and antigen-specific IgE levels in serum and gene expression of selected cytokines in peripheral blood were measured in 386 children aged 1\u20138 years. TaqMan allelic discrimination, amplification refractory mutation system\u2013polymerase chain reaction (ARMS\u2013PCR) and restriction fragment length polymorphisms (RFLP) methods validated by sequencing were used for genotyping. All genotypes for children with total and antigen-specific IgE levels in the normal range were in Hardy\u2013Weinberg equilibrium. Gene expression analyses were carried out using TaqMan gene expression assays. We found elevated total IgE levels in carriers of IL13 Arg130Gln variant allele [odds ratio (OR) = 1\u00b784; 95% confidence interval (CI) = 1\u00b716-2\u00b793]. This effect was more apparent for boys (OR = 2\u00b731; 95% CI = 1\u00b725-4\u00b728). However, no significant association was observed for the other four variants examined. We found up-regulation of IFN-\u03b3 in children with elevated serum total IgE levels carrying the Arg130 allele (P\u2009=\u20090\u00b7005). No differences were found for IL4, IL8 or IL10, while IL13 gene expression was under the detection limit. IL13 Arg130Gln genotypes can play a role in genetic susceptibility to allergy via regulation of serum total IgE levels and affecting IFN-\u03b3 gene expression.<\/jats:p>","DOI":"10.1111\/cei.12923","type":"journal-article","created":{"date-parts":[[2017,1,5]],"date-time":"2017-01-05T09:05:50Z","timestamp":1483607150000},"page":"45-52","source":"Crossref","is-referenced-by-count":10,"title":["Impact of interleukin 13 (<i>IL13<\/i>) genetic polymorphism Arg130Gln on total serum immunoglobulin (IgE) levels and interferon (IFN)-\u03b3 gene expression"],"prefix":"10.1093","volume":"188","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4906-5652","authenticated-orcid":false,"given":"B","family":"Smolkova","sequence":"first","affiliation":[{"name":"Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia"},{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Tulinska","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Palkovicova Murinova","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Buocikova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Liskova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Rausova","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Kuricova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Patayova","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Sustrova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Neubauerova Svorcova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Ilavska","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Szabova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Nemessanyi","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Jahnova","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Dusinska","sequence":"additional","affiliation":[{"name":"Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute of Air Research, Kjeller, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Ciznar","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, 1st Pediatric Department, Comenius University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Fuortes","sequence":"additional","affiliation":[{"name":"College of Public Health, University of Iowa, Iowa City, IA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,2,7]]},"reference":[{"key":"2021120808084922100_cei12923-bib-0001","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/sj.gene.6364284","article-title":"Asthma genetics 2006: the long and winding road to gene discovery","volume":"7","author":"Ober","year":"2006","journal-title":"Genes 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