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In the absence of ZAP-70, T cell development is arrested in the CD4+CD8+ double-positive stage, thus ZAP-70 homozygous knockout (ZAP-70\u2212\/\u2212) mice have no mature T cells in their peripheral lymphoid organs and blood, causing severe immunodeficiency. We investigated the early kinetics and long-term effects of wild-type thymocyte transfer on T cell repopulation in ZAP-70\u2212\/\u2212 mice. We used a single intraperitoneal (i.p.) injection to deliver donor thymocytes to the recipients. Here, we show that after i.p. injection donor thymocytes leave the peritoneum through milky spots in the omentum and home to the thymus, where donor-originated CD4\u2212CD8\u2212 double-negative thymocytes most probably restore T cell development and the disrupted thymic architecture. Subsequently, newly developed, donor-originated, single-positive \u03b1\u03b2 T cells appear in peripheral lymphoid organs, where they form organized T cell zones. The established chimerism was found to be stable, as donor-originated cells were present in transferred ZAP-70\u2212\/\u2212 mice as late as 8 months after i.p. injection. We demonstrate that a simple i.p. injection of ZAP-70+\/+ thymocytes is a feasible method for the long-term reconstitution of T cell development in ZAP-70-deficient mice.<\/jats:p>","DOI":"10.1111\/cei.13114","type":"journal-article","created":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T16:02:38Z","timestamp":1518451358000},"page":"302-314","source":"Crossref","is-referenced-by-count":0,"title":["Correction of T cell deficiency in ZAP-70 knock-out mice by simple intraperitoneal adoptive transfer of thymocytes"],"prefix":"10.1093","volume":"192","author":[{"given":"R","family":"Kugyelka","sequence":"first","affiliation":[{"name":"Department of Immunology and Biotechnology, Medical School, University of Pecs, Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z","family":"Kohl","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Medical School, University of Pecs, Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Olasz","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Medical School, University of Pecs, Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Prenek","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Medical School, University of Pecs, Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Berki","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Medical School, University of Pecs, Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Balogh","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Medical School, University of Pecs, Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7831-1018","authenticated-orcid":false,"given":"F","family":"Boldizs\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Medical School, University of Pecs, Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,3,12]]},"reference":[{"key":"2021120808313581600_cei13114-bib-0001","doi-asserted-by":"crossref","first-page":"9166","DOI":"10.1073\/pnas.88.20.9166","article-title":"The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70, a 70-kDa tyrosine phosphoprotein","volume":"88","author":"Chan","year":"1991","journal-title":"Proc Natl Acad Sci USA"},{"key":"2021120808313581600_cei13114-bib-0002","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0092-8674(93)90221-B","article-title":"T cell 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