{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:23:24Z","timestamp":1784247804907,"version":"3.55.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":777,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,15]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Vaccination against meningococcal serogroup B is recommended for patients with a complement deficiency; however, although immunogenicity in this patient group has been shown, efficacy has not yet been established. In this study, we collected serum from children with a complement deficiency in the alternative pathway or in late terminal pathway before and after vaccination with multi-component meningococcal serogroup B (MenB)-4C. MenB-4C is a multi-component, protein-based vaccine against MenB consisting of factor H-binding protein, Neisserial heparin-binding protein, Neisserial adhesion A and outer membrane vesicles containing Porin A. We assessed the vaccine immunogenicity and vaccine-mediated protection by a whole cell enzyme-linked immunosorbent assay with Neisseria meningitidis serogroup B strains H44\/76, 5\/99 and NZ98\/254, which shows that vaccination induced antibody titers against meningococcus. We show that the classical serum bactericidal activity assay with exogenous serum indicates the presence of vaccine-induced antibodies and capacity to activate complement-mediated pathogen lysis. However, in children with a late terminal pathway deficiency, no complement-mediated pathogen lysis was observed when autologous serum was applied in the serum bactericidal activity assay, demonstrating a lack of serum bactericidal activity in children with complement deficiencies. However, MenB-4C vaccination still induced effective complement-dependent opsonophagocytic killing against N. meningitidis serogroup B in reconstituted whole blood with autologous serum from children with an alternative pathway or late terminal pathway deficiency. These findings support the recommendation to vaccinate all complement-deficient children against MenB.<\/jats:p>","DOI":"10.1111\/cei.13368","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T17:28:30Z","timestamp":1567704510000},"page":"381-389","source":"Crossref","is-referenced-by-count":16,"title":["Multi-component meningococcal serogroup B (MenB)-4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency"],"prefix":"10.1093","volume":"198","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0192-4788","authenticated-orcid":false,"given":"B","family":"van den Broek","sequence":"first","affiliation":[{"name":"Pediatric Infectious Diseases and Immunology, Amalia Children\u2019s Hospital, Nijmegen, the Netherlands"},{"name":"Expertise Center for Immunodeficiency and Autoinflammation (REIA), Radboudumc, Nijmegen, the Netherlands"},{"name":"Section Pediatric Infectious Diseases, Radboud Institute of Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands"},{"name":"Radboud Center for Infectious Diseases, Radboudumc, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C A C M","family":"van Els","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Control, National Institute of Public Health and the Environment (RIVM), Bilthoven, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Control, National Institute of Public Health and the Environment (RIVM), Bilthoven, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"van Aerde","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases and Immunology, Amalia Children\u2019s Hospital, Nijmegen, the Netherlands"},{"name":"Expertise Center for Immunodeficiency and Autoinflammation (REIA), Radboudumc, Nijmegen, the Netherlands"},{"name":"Section Pediatric Infectious Diseases, Radboud Institute of Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands"},{"name":"Radboud Center for Infectious Diseases, Radboudumc, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S S","family":"Henriet","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases and Immunology, Amalia Children\u2019s Hospital, Nijmegen, the Netherlands"},{"name":"Expertise Center for Immunodeficiency and Autoinflammation (REIA), Radboudumc, Nijmegen, the Netherlands"},{"name":"Section Pediatric Infectious Diseases, Radboud Institute of Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands"},{"name":"Radboud Center for Infectious Diseases, Radboudumc, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"de Groot","sequence":"additional","affiliation":[{"name":"Section Pediatric Infectious Diseases, Radboud Institute of Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands"},{"name":"Radboud Center for Infectious Diseases, Radboudumc, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M I","family":"de Jonge","sequence":"additional","affiliation":[{"name":"Section Pediatric Infectious Diseases, Radboud Institute of Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands"},{"name":"Radboud Center for Infectious Diseases, Radboudumc, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5317-3202","authenticated-orcid":false,"given":"J D","family":"Langereis","sequence":"additional","affiliation":[{"name":"Section Pediatric Infectious Diseases, Radboud Institute of Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands"},{"name":"Radboud Center for Infectious Diseases, Radboudumc, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"van der Flier","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases and Immunology, Amalia Children\u2019s Hospital, Nijmegen, the Netherlands"},{"name":"Expertise Center for Immunodeficiency and Autoinflammation (REIA), Radboudumc, Nijmegen, the Netherlands"},{"name":"Section Pediatric Infectious Diseases, Radboud Institute of Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands"},{"name":"Radboud Center for Infectious Diseases, Radboudumc, Nijmegen, the Netherlands"},{"name":"Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, the 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