{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T17:10:15Z","timestamp":1788196215509,"version":"build-2803163510"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,12,27]],"date-time":"2020-12-27T00:00:00Z","timestamp":1609027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Autoimmune neutropenia (AIN) in childhood is characterized by chronic neutropenia and positivity for anti-neutrophil antibodies, resulting in the excessive destruction of neutrophils. In this study, we investigated the involvement of regulatory T cells (Tregs) in the pathogenesis of AIN in childhood. Tregs have been classified into three subpopulations based on the expressions of CD45RA and forkhead box protein 3 (FoxP3): resting Tregs, activated Tregs and non-suppressive Tregs. The frequency of activated Tregs (CD4+CD25+FoxP3highCD45RA\u2212 T cells) as well as that of total Tregs (CD4+CD25+FoxP3+ T cells) in peripheral blood was significantly decreased in patients with AIN. Analysis of the T cell receptor (TCR)-V\u03b2 repertoire of CD4+ T cells revealed skewed usages in patients with AIN compared with that observed in age-matched control subjects. Regarding T cell subsets, the use of four of 24 TCR-V\u03b2 families in Tregs and one in conventional T cells were increased in patients with AIN. The number of patients with AIN who showed skewed usages of TCR-V\u03b2 family in conventional and Tregs was significantly higher than that reported in control subjects. When the preference between Tregs and conventional T cells in each TCR-V\u03b2 family was individually compared, different use was prominently observed in the TCR-V\u03b2 9 family in patients with AIN. These results suggest that the quantitative abnormalities of Tregs and the skew of the TCR-V\u03b2 repertoire in CD4+ T cells, including Tregs and conventional T cells, may be related to autoantibody production through a human neutrophil antigen-reactive T cell clone.<\/jats:p>","DOI":"10.1111\/cei.13559","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T21:46:59Z","timestamp":1606945619000},"page":"1-13","source":"Crossref","is-referenced-by-count":9,"title":["Possible involvement of regulatory T cell abnormalities and variational usage of TCR repertoire in children with autoimmune neutropenia"],"prefix":"10.1093","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0135-8701","authenticated-orcid":false,"given":"S","family":"Goda","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Hiroshima University Graduate School of Biomedical and Health Sciences , Hiroshima, 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