{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:08:35Z","timestamp":1790752115511,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T00:00:00Z","timestamp":1372723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Adolescents with anorexia nervosa (<jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content>) are amenorrheic and have decreased bone mass accrual and low bone mineral density (<jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content>). The regulation of mesenchymal stem cell differentiation is an important factor governing bone formation. Preadipocyte factor 1 (Pref\u20101), an inhibitor of adipocyte and osteoblast differentiation, is elevated in states of oestrogen deficiency. In this study, we aim to (i) investigate effects of transdermal oestradiol on Pref\u20101 in adolescent girls with <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content>, and (ii) examine associations of changes in Pref\u20101 with changes in lumbar <jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content> and bone turnover markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Adolescent girls with <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content> and normal\u2010weight controls were studied cross\u2010sectionally. Girls with <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content> were examined longitudinally in a double\u2010blind study and received transdermal oestradiol (plus cyclic medroxyprogesterone) or placebo for 12\u00a0months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients<\/jats:title><jats:p>Sixty\u2010nine girls (44 with <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content>, 25 normal\u2010weight controls) 13\u201318\u00a0years were studied at baseline; 22 <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content> girls were followed prospectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurements<\/jats:title><jats:p>Pref\u20101 levels, bone formation and resorption markers, and <jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pref\u20101 levels decreased in girls with <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content> after treatment with transdermal oestradiol compared with placebo (\u22120\u00b7015\u00a0\u00b1\u00a00\u00b7016 <jats:italic>vs<\/jats:italic> 0\u00b7060\u00b10\u00b7026\u00a0ng\/ml, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b701), although at baseline, levels did not differ in <jats:styled-content style=\"fixed-case\">AN <\/jats:styled-content><jats:italic>vs<\/jats:italic> controls (0\u00b7246\u00a0\u00b1\u00a00\u00b7015 <jats:italic>vs<\/jats:italic> 0\u00b7267\u00a0\u00b1\u00a00\u00b7022\u00a0ng\/ml). Changes in Pref\u20101 over 12\u00a0months correlated inversely with changes in lumbar <jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content> (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120\u00b748, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b702) and positively with changes in <jats:styled-content style=\"fixed-case\">CTX<\/jats:styled-content> (<jats:italic>r<\/jats:italic>\u00a0=\u00a00\u00b773, <jats:italic>P\u00a0<\/jats:italic>=\u00a00\u00b7006).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For the first time, we show that Pref\u20101 is negatively regulated by oestradiol in adolescent girls with <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content>. Inhibition of Pref\u20101 may mediate the beneficial effects of transdermal oestradiol replacement on <jats:styled-content style=\"fixed-case\">BMD<\/jats:styled-content> in girls with <jats:styled-content style=\"fixed-case\">AN<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.12144","type":"journal-article","created":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T14:41:06Z","timestamp":1358433666000},"page":"326-332","source":"Crossref","is-referenced-by-count":36,"title":["Inhibition of Pref\u20101 (preadipocyte factor 1) by oestradiol in adolescent girls with anorexia nervosa is associated with improvement in lumbar bone mineral density"],"prefix":"10.1111","volume":"79","author":[{"given":"Alexander T.","family":"Faje","sequence":"first","affiliation":[{"name":"Neuroendocrine Unit Massachusetts General Hospital for Children and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pouneh 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