{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:26:31Z","timestamp":1791300391135,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,5,20]],"date-time":"2013-05-20T00:00:00Z","timestamp":1369008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Kisspeptin is a novel hypothalamic peptide which stimulates endogenous gonadotrophin releasing hormone (GnRH) secretion. A single subcutaneous bolus injection of kisspeptin\u201054 increases circulating luteinizing hormone (LH) levels in women, but its acute effects on LH pulsatility are not known.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the effects of a single subcutaneous (sc) injection of kisspeptin\u201054 administration on LH pulsatility in healthy female volunteers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six healthy female adult volunteers underwent 10\u2010minute blood sampling for serum LH measurement for 8\u00a0h during the follicular phase of menstrual cycle. Sc bolus injection of saline or kisspeptin\u201054 (0\u00b715, 0\u00b730 or 0\u00b760\u00a0nmol\/kg) was administered 4\u00a0h after commencing the study. A previously described, blinded deconvolution method was used to detect LH pulses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean number of LH pulses was increased significantly following 0\u00b730 and 0\u00b760\u00a0nmol\/kg kisspeptin\u201054 when compared with saline (mean increase in number of LH pulses per 4\u00a0h, following injection: \u22120\u00b717\u00a0\u00b1\u00a00\u00b754, saline; +2\u00b733\u00a0\u00b1\u00a00\u00b756, 0\u00b730\u00a0nmol\/kg kisspeptin\u201054, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b705 <jats:italic>vs<\/jats:italic> saline; +2\u00b733\u00a0\u00b1\u00a00\u00b780, 0\u00b760\u00a0nmol\/kg kisspeptin\u201054, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b705 <jats:italic>vs<\/jats:italic> saline). LH pulse secretory mass increased following injection of 0\u00b760\u00a0nmol\/kg in five of six subjects, but the mean change in all subjects was non\u2010significant when compared with saline (mean increase in pulse secretory mass in IU\/l following injection: +0\u00b735\u00a0\u00b1\u00a00\u00b740, saline; +2\u00b761\u00a0\u00b1\u00a01\u00b717, 0\u00b760\u00a0nmol\/kg kisspeptin\u201054, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b710 <jats:italic>vs<\/jats:italic> saline).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A single injection of kisspeptin\u201054 temporarily stimulates the number of LH pulses in healthy women. Further studies are required to investigate the therapeutic potential of kisspeptin\u201054 injection to restore LH pulsatility in patients with reproductive disorders caused by impaired GnRH secretion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.12179","type":"journal-article","created":{"date-parts":[[2013,3,2]],"date-time":"2013-03-02T17:13:32Z","timestamp":1362244412000},"page":"558-563","source":"Crossref","is-referenced-by-count":54,"title":["A single injection of kisspeptin\u201054 temporarily increases luteinizing hormone pulsatility in healthy women"],"prefix":"10.1111","volume":"79","author":[{"given":"C. N.","family":"Jayasena","sequence":"first","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. N.","family":"Comninos","sequence":"additional","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. D.","family":"Veldhuis","sequence":"additional","affiliation":[{"name":"Endocrine Research Unit Center for Translational Science Activities Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Misra","sequence":"additional","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"},{"name":"Department of Clinical Biochemistry Imperial College Healthcare NHS Trust Charing Cross Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Abbara","sequence":"additional","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Izzi\u2010Engbeaya","sequence":"additional","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Donaldson","sequence":"additional","affiliation":[{"name":"Endocrine Research Unit Center for Translational Science Activities Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Ghatei","sequence":"additional","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. R.","family":"Bloom","sequence":"additional","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. S.","family":"Dhillo","sequence":"additional","affiliation":[{"name":"Section of Investigative Medicine Imperial College London Hammersmith Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/frne.2001.0211"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104847200"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834399100"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa035322"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa073443"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.05.185"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409330102"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409822102"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-1261"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.09.021"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000083140"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0787"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3328-05.2005"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5740-08.2009"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-1468"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1116"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-0406"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2010.204"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-0089"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000336376"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2012-1282"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des326"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-1408"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-3046"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2007.093005"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00071.2009"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-7-1-11"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcen.12179","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.12179","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T17:47:40Z","timestamp":1696355260000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cen.12179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,20]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1111\/cen.12179"],"URL":"https:\/\/doi.org\/10.1111\/cen.12179","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,20]]}}}