{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T01:19:51Z","timestamp":1783732791845,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,3,19]],"date-time":"2014-03-19T00:00:00Z","timestamp":1395187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Context<\/jats:title><jats:p>Autoimmune thyroid diseases (<jats:styled-content style=\"fixed-case\">AITDs<\/jats:styled-content>), including Graves\u2019 disease (<jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content>) and Hashimoto's thyroiditis (<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content>), are the most common autoimmune diseases. Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are small noncoding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s, which can play pivotal roles in immune functions and development of autoimmunity. Recently, it has been recognized that identification of circulating mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s can provide important and novel information regarding disease pathogenesis and clinical condition. However, the role circulating mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">AITD<\/jats:styled-content> has not yet been described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to characterize the different circulating levels of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in patients with <jats:styled-content style=\"fixed-case\">AITD<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design and methods<\/jats:title><jats:p>Sixty\u2010four participants who met the criteria for <jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content> and healthy subjects were recruited. Microarrays were used to analyse the expression patterns of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in serum obtained from patients with <jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content> and healthy subjects. After analysing the microarray data, four interesting mi<jats:styled-content style=\"fixed-case\">RNAs<\/jats:styled-content> (miR\u201016, miR\u201022, miR\u2010375 and miR\u2010451) were selected and validated by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were observed to be differently expressed in serum from patients with <jats:styled-content style=\"fixed-case\">AITD<\/jats:styled-content> compared with healthy subjects by microarray analysis. Further analysis consistently showed that serum levels of miR\u201022, miR\u2010375 and miR\u2010451 were increased in patients with <jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content>. On the other hand, the serum levels of miR\u201016, miR\u201022, miR\u2010375 and miR\u2010451 were increased in patients with <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content> compared with healthy subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We revealed that different levels of serum mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were associated with <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content>, which may play a role in the pathogenesis of these diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.12432","type":"journal-article","created":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T14:52:05Z","timestamp":1392648725000},"page":"276-281","source":"Crossref","is-referenced-by-count":79,"title":["Circulating micro<scp>RNA<\/scp>s in autoimmune thyroid diseases"],"prefix":"10.1111","volume":"81","author":[{"given":"Hiroya","family":"Yamada","sequence":"first","affiliation":[{"name":"Department of Hygiene Fujita Health University School of Medicine Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuyasu","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Endocrinology &amp; Metabolism Fujita Health University School of Medicine Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izumi","family":"Hiratsuka","sequence":"additional","affiliation":[{"name":"Department of Endocrinology &amp; Metabolism Fujita Health University School of Medicine Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Hygiene Fujita Health University School of Medicine Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI14321"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-2982"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2708"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2012.0277"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2011.76"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.05.021"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-3059"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2012.04.002"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31822200cc"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-011-1441-7"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1918"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01875-08"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101578"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-012-8330-2"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-1719"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2008.0423"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201165"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-012-8264-z"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-13-116"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916930310001603046"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2010.04306.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2009.03884.x"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcen.12432","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.12432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T10:50:27Z","timestamp":1696330227000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cen.12432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,19]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/cen.12432"],"URL":"https:\/\/doi.org\/10.1111\/cen.12432","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,19]]}}}