{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T05:10:57Z","timestamp":1695618657184},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T00:00:00Z","timestamp":1402531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Medical University of Warsaw","award":["1WY\/N\/13"],"award-info":[{"award-number":["1WY\/N\/13"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>T\u2010cell receptor rearrangement excision circles (<jats:styled-content style=\"fixed-case\">TREC<\/jats:styled-content>) are circular <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> molecules generated during T\u2010cell maturation in the thymus. Recent studies suggested that a decreased <jats:styled-content style=\"fixed-case\">TREC<\/jats:styled-content> concentration in peripheral blood may be a general feature of autoimmunity. Our purpose was to assess the <jats:styled-content style=\"fixed-case\">TREC<\/jats:styled-content> concentration in Graves' disease (<jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">TREC<\/jats:styled-content> concentration was assessed by real time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples isolated from peripheral blood leucocytes among younger (<jats:italic>n<\/jats:italic>\u00a0=\u00a094, age range 6\u201329\u00a0years) and older patients with <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a093, age range 57\u201380\u00a0years) and age\u2010matched controls (<jats:italic>n<\/jats:italic>\u00a0=\u00a0206).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">TREC<\/jats:styled-content> concentration decreased with age in all subjects, but it was significantly higher in <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content> compared with controls (<jats:italic>P<\/jats:italic>\u00a0=\u00a09\u00b74\u00a0\u00d7\u00a010<jats:sup>\u221210<\/jats:sup>). <jats:styled-content style=\"fixed-case\">TREC<\/jats:styled-content> concentration was higher (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b70038) in hyperthyroid (<jats:italic>n<\/jats:italic>\u00a0=\u00a078) than euthyroid (<jats:italic>n<\/jats:italic>\u00a0=\u00a082) patients with <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content>, but in both groups, it remained increased relative to controls (<jats:italic>P<\/jats:italic>\u00a0=\u00a02\u00b72\u00a0\u00d7\u00a010<jats:sup>\u221211<\/jats:sup> and <jats:italic>P<\/jats:italic>\u00a0=\u00a04\u00b74\u00a0\u00d710<jats:sup>\u22127<\/jats:sup>, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Patients with <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content>, particularly those with hyperthyroidism, have increased concentration of <jats:italic>TREC<\/jats:italic> which may suggest increased rather than decreased thymic activity. Thus, <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content> does not follow the paradigm suggested for other autoimmune disorders which links autoimmunity with thymic senescence.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.12492","type":"journal-article","created":{"date-parts":[[2014,5,10]],"date-time":"2014-05-10T15:48:46Z","timestamp":1399736926000},"page":"769-774","source":"Crossref","is-referenced-by-count":3,"title":["Increased concentration of T\u2010cell receptor rearrangement excision circles (<scp>TREC<\/scp>) in peripheral blood in Graves' disease"],"prefix":"10.1111","volume":"81","author":[{"given":"Katarzyna","family":"Strawa","sequence":"first","affiliation":[{"name":"Department of Medical Genetics Centre for Biostructure Medical University of Warsaw Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Markowska","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics Centre for Biostructure 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