{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T03:07:47Z","timestamp":1782961667019,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,22]],"date-time":"2014-09-22T00:00:00Z","timestamp":1411344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background\/objective<\/jats:title><jats:p>There is an increasing rate of papillary thyroid carcinomas that may never progress to cause symptoms or death. Predicting outcome and determining tumour aggressiveness could help diminish the number of patients submitted to aggressive treatments. We aimed to evaluate whether markers of the immune system response and of tumour\u2010associated inflammation could predict outcome of differentiated thyroid cancer (<jats:styled-content style=\"fixed-case\">DTC<\/jats:styled-content>) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Retrospective cohort study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients<\/jats:title><jats:p>We studied 399 consecutive patients, including 325 papillary and 74 follicular thyroid carcinomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurements<\/jats:title><jats:p>Immune cell markers were evaluated using immunohistochemistry, including tumour\u2010associated macrophages (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68) and subsets of tumour\u2010infiltrating lymphocytes (<jats:styled-content style=\"fixed-case\">TIL<\/jats:styled-content>), such as <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>16, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>20, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45<jats:styled-content style=\"fixed-case\">RO<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> GRANZYME<\/jats:styled-content> B, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>69 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25. We also investigated the expression of cyclooxygenase 2 (<jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>2) in tumour cells and the presence of concurrent lymphocytic infiltration characterizing chronic thyroiditis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Concurrent lymphocytic infiltration characterizing chronic thyroiditis was observed in 29% of the cases. Among all the immunological parameters evaluated, only the enrichment of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8+ lymphocytes (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7001) and expression of <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>2 (<jats:italic>P<\/jats:italic>\u00a0=0\u00b701) were associated with recurrence. A multivariate model analysis identified <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8+ <jats:styled-content style=\"fixed-case\">TIL<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>2 as independent risk factor for recurrence. A multivariate analysis using Cox's proportional\u2010hazards model adjusted for the presence of concurrent chronic thyroiditis demonstrated that the presence of concurrent chronic thyroiditis had no effect on prognostic prediction mediated by <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8+ <jats:styled-content style=\"fixed-case\">TIL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, we suggest the use of a relatively simple pathology tool to help select cases that may benefit of a more aggressive approach sparing the majority of patients from unnecessary procedures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.12586","type":"journal-article","created":{"date-parts":[[2014,8,16]],"date-time":"2014-08-16T06:09:08Z","timestamp":1408169348000},"page":"246-253","source":"Crossref","is-referenced-by-count":60,"title":["<scp>CD<\/scp>8+ tumour\u2010infiltrating lymphocytes and COX2 expression may predict relapse in differentiated thyroid cancer"],"prefix":"10.1111","volume":"83","author":[{"given":"Lucas Leite","family":"Cunha","sequence":"first","affiliation":[{"name":"Laboratory of Cancer Molecular Genetics Faculty of Medical Sciences \u2013 University of Campinas (Unicamp) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjory Alana","family":"Marcello","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Molecular Genetics Faculty of Medical Sciences \u2013 University of Campinas (Unicamp) Campinas 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