{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T02:44:30Z","timestamp":1748745870623},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000858","name":"University of Sheffield","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"Summary<\/jats:title>Objectives<\/jats:title>Cytokines have an important role in orchestrating the pathophysiology in autoimmune thyroid disease. The aim of the current study was to analyse the expression of interleukin (IL<\/jats:styled-content>)\u201014 and IL<\/jats:styled-content>\u201016 in the thyroid tissue of patients with Graves\u2019 disease (GD<\/jats:styled-content>), Hashimoto's thyroiditis (HT<\/jats:styled-content>) or multinodular goitre (MNG<\/jats:styled-content>) and in that of normal individuals, in patients\u2019 intrathyroidal CD<\/jats:styled-content>4+<\/jats:sup> and CD<\/jats:styled-content>8+<\/jats:sup> T cells, and in patient and normal cultured thyroid follicular cells.<\/jats:p><\/jats:sec>Methods<\/jats:title>The expression of IL<\/jats:styled-content>\u201014 and IL<\/jats:styled-content>\u201016 mRNA<\/jats:styled-content> and protein was investigated using reverse transcription\u2010polymerase chain reaction (RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>) amplification, and Western blotting and ELISA<\/jats:styled-content>s, respectively.<\/jats:p><\/jats:sec>Results<\/jats:title>IL<\/jats:styled-content>\u201014 mRNA<\/jats:styled-content> expression was detected in thyroid tissue from 8\/9 GD<\/jats:styled-content>, 3\/4 HT<\/jats:styled-content> and 3\/13 MNG<\/jats:styled-content> patients and 1\/6 normal individuals, and IL<\/jats:styled-content>\u201016 mRNA<\/jats:styled-content> expression in thyroid tissue from 9\/9 GD<\/jats:styled-content>, 4\/4 HT<\/jats:styled-content> and 9\/13 MNG<\/jats:styled-content> patients and 4\/6 normal individuals. IL<\/jats:styled-content>\u201014 mRNA<\/jats:styled-content> expression was detected in intrathyroidal CD<\/jats:styled-content>4+<\/jats:sup> and CD<\/jats:styled-content>8+<\/jats:sup> T cells from 2\/2 GD<\/jats:styled-content> and 2\/2 HT<\/jats:styled-content> patients, while IL<\/jats:styled-content>\u201016 mRNA<\/jats:styled-content> was detected in samples from 1\/2 HT patients<\/jats:styled-content> but not in those from either patient with GD<\/jats:styled-content>. IL<\/jats:styled-content>\u201014 and IL<\/jats:styled-content>\u201016 mRNA<\/jats:styled-content> expression was found in thyroid follicular cells derived from 2\/2 patient with GD<\/jats:styled-content> and 1\/1 normal individual. IL<\/jats:styled-content>\u201014 protein was detected in thyroid tissue from 6\/6 GD<\/jats:styled-content>, 1\/1 HT<\/jats:styled-content> and 0\/6 MNG<\/jats:styled-content> patients and 0\/6 normal individuals, and IL<\/jats:styled-content>\u201016 protein in thyroid tissue from 6\/6 GD<\/jats:styled-content>, 1\/1 HT<\/jats:styled-content> and 1\/6 MNG<\/jats:styled-content> patients and 0\/6 normal individuals. Expression of IL<\/jats:styled-content>\u201014 protein was stimulated in thyroid follicular cells derived from two patients with GD<\/jats:styled-content> and one normal individual by peripheral blood mononuclear cell (PBMC<\/jats:styled-content>)\u2010conditioned medium. Treatment of thyrocytes from two patients with GD<\/jats:styled-content> and one normal individual with PBMC<\/jats:styled-content>\u2010conditioned medium and tumour necrosis factor (TNF<\/jats:styled-content>)\u2010\u03b1 stimulated IL<\/jats:styled-content>\u201016 protein expression. In normal thyrocytes, IL<\/jats:styled-content>\u201016 protein synthesis was induced also by IL<\/jats:styled-content>\u20101\u03b2, IL<\/jats:styled-content>\u201017A, IL<\/jats:styled-content>\u20104 and transforming growth factor (TGF)\u2010\u03b2.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The data provide evidence that the intrathyroidal production of IL<\/jats:styled-content>\u201014 and IL<\/jats:styled-content>\u201016 is associated with the pathogenesis of autoimmune thyroid disease. Thyroid follicular cells display the ability to express IL<\/jats:styled-content>\u201014 and IL<\/jats:styled-content>\u201016 mRNA<\/jats:styled-content> and can be stimulated to express IL<\/jats:styled-content>\u201016 protein, by a panel of cytokines, and IL<\/jats:styled-content>\u201014 protein, by as yet unidentified factors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.12810","type":"journal-article","created":{"date-parts":[[2015,5,4]],"date-time":"2015-05-04T14:22:06Z","timestamp":1430749326000},"page":"726-732","source":"Crossref","is-referenced-by-count":11,"title":["IL<\/scp>\u201014 and IL<\/scp>\u201016 are expressed in the thyroid of patients with either Graves\u2019 disease or Hashimoto's thyroiditis"],"prefix":"10.1111","volume":"83","author":[{"given":"Elizabeth","family":"Helen Kemp","sequence":"first","affiliation":[{"name":"Department of Human Metabolism The Medical School University of Sheffield Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramzi A.","family":"Ajjan","sequence":"additional","affiliation":[{"name":"Leeds Institute for Cardiovascular and Metabolic Medicine University of Leeds Leeds UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russell A.","family":"Metcalfe","sequence":"additional","affiliation":[{"name":"Department of Human Metabolism The Medical School University of Sheffield Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip F.","family":"Watson","sequence":"additional","affiliation":[{"name":"Department of Human Metabolism The Medical School University of Sheffield Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony P.","family":"Weetman","sequence":"additional","affiliation":[{"name":"Department of Human Metabolism The Medical School University of Sheffield Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e3280113d1a"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1480001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2002-0020"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/378588"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2011.0049"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.82.2.666"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2012007500168"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.5.2470"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2613.2010.00715.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.13.6330"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2008.10.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.8.5676"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5109"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916930108994108"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199809)28:09<2661::AID-IMMU2661>3.0.CO;2-N"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2000.00997.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.21.6.3671"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000024186"},{"key":"e_1_2_7_20_1","first-page":"12","article-title":"Interleukin\u201016 expression in relation to disease activity in rheumatoid arthritis","volume":"28","author":"Blaschke S.","year":"2001","journal-title":"Journal of Rheumatology"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.6.795"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.48.3.326"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0199"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-1375"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.d01-784.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1989.387"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-61-5-817"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0150011"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2443.2003.00612.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.5273"},{"key":"e_1_2_7_31_1","first-page":"196","article-title":"Thyroid antibodies are produced by thyroid\u2010derived lymphocytes","volume":"48","author":"Weetman A.P.","year":"1982","journal-title":"Clinical and Experimental Immunology"},{"key":"e_1_2_7_32_1","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.4049\/jimmunol.162.3.1287","article-title":"Processing and release of IL\u201016 from CD41 but Not CD81 T cells is activation dependent","volume":"162","author":"Wu D.M.","year":"1999","journal-title":"Journal of Immunology"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.7.3806"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3858\/emm.2008.40.2.237"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcen.12810","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.12810","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T09:23:15Z","timestamp":1695547395000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cen.12810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/cen.12810"],"URL":"https:\/\/doi.org\/10.1111\/cen.12810","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6]]}}}