{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:47:24Z","timestamp":1788306444212,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,10,9]],"date-time":"2015-10-09T00:00:00Z","timestamp":1444348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>As nonclassic congenital adrenal hyperplasia (<jats:styled-content style=\"fixed-case\">NCCAH<\/jats:styled-content>) needs to be taken into account in women with hyperandrogenism, we aimed to assess whether the recommended level of poststimulated 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> \u226530 nmol\/l confirms <jats:styled-content style=\"fixed-case\">NCCAH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>Forty, consecutive women with biochemical and\/or clinical hyperandrogenism (aged 25\u00b74, 18\u201338) suspected of having <jats:styled-content style=\"fixed-case\">NCCAH<\/jats:styled-content> were recruited to the study. In patients with 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> level between 5\u00b71 and 29\u00b79 nmol\/l an <jats:styled-content style=\"fixed-case\">ACTH<\/jats:styled-content> stimulation test was performed. In patients with basal or poststimulated 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> \u226530 nmol\/l, twenty\u2010four\u2010hour urinary steroid profile (<jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content>) analysis was performed and <jats:italic><jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>21A<\/jats:italic>2 mutation was assessed. In selected patients with poststimulated 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> &lt;30 nmol\/l <jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content> was also performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The group was divided into two subgroups with basal or poststimulated 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> \u226530 nmol\/l (group A) and with poststimulated 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> &lt;30 nmol\/l (group B). Among 40 patients, basal or poststimulated 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> \u226530 nmol\/l was found in 21, but <jats:styled-content style=\"fixed-case\">NCCAH<\/jats:styled-content> was confirmed by <jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content> followed by genetic testing only in 5 (24%). Four patients were diagnosed as heterozygotes, and in twelve, no <jats:italic><jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>21A2<\/jats:italic> mutation was detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The diagnosis of <jats:styled-content style=\"fixed-case\">NCCAH<\/jats:styled-content> based only on serum 17<jats:styled-content style=\"fixed-case\">OHP<\/jats:styled-content> measurements (basal or poststimulated) may lead to false\u2010positive diagnosis when performed by immunoassay with a cut\u2010off value of \u226530 nmol\/l. The definitive diagnosis can be established based on <jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content> and\/or genetic testing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.12935","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T16:05:44Z","timestamp":1441209944000},"page":"23-29","source":"Crossref","is-referenced-by-count":30,"title":["The diagnosis of nonclassic congenital adrenal hyperplasia due to 21\u2010hydroxylase deficiency, based on serum basal or post\u2010<scp>ACTH<\/scp> stimulation 17\u2010hydroxyprogesterone, can lead to false\u2010positive diagnosis"],"prefix":"10.1111","volume":"84","author":[{"given":"Urszula","family":"Ambroziak","sequence":"first","affiliation":[{"name":"Department of Internal Medicine and Endocrinology Medical University of Warsaw Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"K\u0119pczy\u0144ska\u2010Nyk","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Endocrinology Medical University of Warsaw Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Kury\u0142owicz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Endocrinology Medical University of Warsaw Warsaw Poland"},{"name":"Department of Human Epigenetics Mossakowski Medical Research Centre Polish Academy of Sciences Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa Maria","family":"Ma\u0142unowicz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Radioimmunology and Experimental Medicine Children's Memorial Health Institute Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"W\u00f3jcicka","sequence":"additional","affiliation":[{"name":"Genomic Medicine Medical University of Warsaw Warsaw Poland"},{"name":"Laboratory of Human Cancer Genetics Centre of New Technologies, CENT University of Warsaw Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Mi\u015bkiewicz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Endocrinology Medical University of Warsaw Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Macech","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Endocrinology Medical University of Warsaw Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2006.02.004"},{"key":"e_1_2_5_3_1","first-page":"142","article-title":"Congenital adrenal hyperplasia due to 21\u2010hydroxylase deficiency \u2013 management in adults","volume":"61","author":"Ambroziak U.","year":"2010","journal-title":"Endokrynologia Polska"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041536"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-2631"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-2053"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(99)00383-0"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-35388"},{"key":"e_1_2_5_9_1","first-page":"9","article-title":"Nonclassic congenital adrenal hyperplasia due to 21\u2010hydroxylase deficiency in girls and women with androgenization symptoms","volume":"1","author":"Malunowicz E.M.","year":"2008","journal-title":"Pediatric Endocrinology"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(93)90132-G"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-21-11-1440"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000185529"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(86)90007-3"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.3.1215"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh098"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-2279"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.51.31"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.2.8247"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-37497"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2000.108020"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008-1582"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.78.4.810"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1515\/jpm-2013-0154"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004563214530676"},{"key":"e_1_2_5_25_1","first-page":"1","article-title":"LC\u2010MS\/MS improves screening towards 21\u2010hydroxylase deficiency","volume":"24","author":"Ambroziak U.","year":"2014","journal-title":"Gynecological Endocrinology"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s100570050001"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00442691"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2004.02.006"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300057110"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046688"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth0410-248"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-032046"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcen.12935","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.12935","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T14:25:40Z","timestamp":1695479140000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cen.12935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,9]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1111\/cen.12935"],"URL":"https:\/\/doi.org\/10.1111\/cen.12935","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,9]]}}}