{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:17:25Z","timestamp":1783487845460,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,2,25]],"date-time":"2016-02-25T00:00:00Z","timestamp":1456358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81130016"],"award-info":[{"award-number":["81130016"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>There are currently no good histological or molecular markers to differentiate benign from malignant phaeochromocytomas and paraganglinomas (PPGLs). Our previous cross\u2010sectional study observed that ERBB\u20102 overexpression was associated with malignant PPGLs. This study aimed to evaluate the predictive value of ERBB\u20102 overexpression for metastasis in PPGLs in a large population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 262 patients diagnosed as PPGLs in our institution between 2002 and 2012 were included. We analysed ERBB\u20102 protein expression in the primary PPGL tumours by immunohistochemistry (IHC) and <jats:italic>ERBB\u20102<\/jats:italic> amplification by fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH). Direct Sanger sequencing was performed to examine <jats:italic>ERBB\u20102<\/jats:italic> exon 20 mutations. The occurrence of malignant PPGLs was documented in the follow\u2010up period. Kaplan\u2013Meier analysis and Cox proportional hazard models were used to evaluate the association between ERBB\u20102 overexpression and metastasis of PPGLs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010six (9\u00b79%) patients had ERBB\u20102 overexpression in their primary PPGL tumours, which was significantly associated with <jats:italic>ERBB\u20102<\/jats:italic> amplification (17\/25, 68%). No <jats:italic>ERBB\u20102<\/jats:italic> mutation was found. At a median follow\u2010up of 4\u00b75 years, a total of 23 malignant PPGLs were documented, including eight (30\u00b78%) patients in the ERBB\u20102 overexpression group and 15 (6\u00b74%) patients in the ERBB\u20102\u2010negative group. The incidence rate of metastasis was 5\u00b73 per 100 person\u2010years <jats:italic>vs<\/jats:italic> 1\u00b74 per 100 person\u2010years in the ERBB\u20102 overexpression and ERBB\u20102\u2010negative groups (<jats:italic>P<\/jats:italic> &lt; 0\u00b7001), respectively. Kaplan\u2013Meier analysis showed that ERBB\u20102 overexpression was associated with decreased metastasis\u2010free survival (<jats:italic>P<\/jats:italic> = 0\u00b7001, log\u2010rank test). After adjusting for primary tumour size and location, Cox regression analysis revealed that ERBB\u20102 overexpression was independently associated with risk of malignant PPGLs (HR = 2\u00b778; 95% CI, 1\u00b712\u20136\u00b790; <jats:italic>P<\/jats:italic> = 0\u00b7028).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Patients harbouring tumours with ERBB\u20102 overexpression have a significantly higher risk of developing malignant PPGLs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.13019","type":"journal-article","created":{"date-parts":[[2016,1,10]],"date-time":"2016-01-10T19:18:20Z","timestamp":1452453500000},"page":"822-829","source":"Crossref","is-referenced-by-count":7,"title":["ERBB\u20102 overexpression as a risk factor for malignant phaeochromocytomas and paraganglinomas"],"prefix":"10.1111","volume":"84","author":[{"given":"Weiqing","family":"Wang","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory for Endocrine Tumors, Shanghai 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Health Ministry Ruijin Hospital, Shanghai Jiao Tong University School of Medicine 197 Ruijin 2nd Road Shanghai 200025 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TingWei","family":"Su","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory for Endocrine Tumors, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of Chinese Health Ministry Ruijin Hospital, Shanghai Jiao Tong University School of Medicine 197 Ruijin 2nd Road Shanghai 200025 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathology Ruijin Hospital, Shanghai Jiao Tong University School of Medicine 197 Ruijin 2nd Road Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Pathology Ruijin Hospital, Shanghai 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