{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T08:51:59Z","timestamp":1768899119815,"version":"3.49.0"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T00:00:00Z","timestamp":1467158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"Summary<\/jats:title>Objective<\/jats:title>It is recognized that measurement of ACTH<\/jats:styled-content>\u2010precursor peptides including proopiomelanocortin (POMC<\/jats:styled-content>) has clinical utility in identifying the aetiology of Cushing's syndrome. Recent data have also demonstrated cross\u2010reactivity of POMC<\/jats:styled-content> in ACTH<\/jats:styled-content> immunoassays used in clinical laboratories. The aim of this study was to assess the cross\u2010reactivity of POMC<\/jats:styled-content> in the main commercial immunoassays for ACTH<\/jats:styled-content> and to survey the awareness of laboratory professionals to this potential interference.<\/jats:p><\/jats:sec>Method<\/jats:title>To assess cross\u2010reactivity, specimens containing ACTH<\/jats:styled-content> and\/or POMC<\/jats:styled-content> were prepared by the UK<\/jats:styled-content> National External Quality Assessment Service (UK NEQAS<\/jats:styled-content>) [Edinburgh]. A separate interpretative exercise was also sent to participating laboratories.<\/jats:p><\/jats:sec>Results<\/jats:title>Eighty\u2010seven laboratories measured \u2018total\u2019 ACTH<\/jats:styled-content> (i.e. ACTH<\/jats:styled-content> and\/or POMC<\/jats:styled-content>) in their assays. Cross\u2010reactivity of POMC<\/jats:styled-content> varied from a mean of 1\u00b76\u20134\u00b77% (reflected in a large percentage increase in measured ACTH<\/jats:styled-content> of up to 261% due to POMC<\/jats:styled-content> cross\u2010reactivity) depending on the manufacturer. Major differences in the clinical interpretation of test results were observed in returned responses to the interpretative exercise.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>An appraisal of POMC<\/jats:styled-content> cross\u2010reactivity in currently available ACTH<\/jats:styled-content> immunoassays has been achieved. Cross\u2010reactivity was sufficient to detect ACTH<\/jats:styled-content> precursors at concentrations that could be found in patients with ectopic ACTH<\/jats:styled-content> syndrome. These data will assist laboratories in interpreting results when assessing the hypothalamic\u2010pituitary\u2010adrenal axis. Endocrinologists and laboratory professionals should be aware of the degree of cross\u2010reactivity in ACTH<\/jats:styled-content> immunoassay in order to minimize the risk of misinterpretation of results and\/or potentially delayed treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.13118","type":"journal-article","created":{"date-parts":[[2016,6,3]],"date-time":"2016-06-03T07:19:59Z","timestamp":1464938399000},"page":"569-574","source":"Crossref","is-referenced-by-count":13,"title":["Proopiomelanocortin interference in the measurement of adrenocorticotrophic hormone: a United Kingdom National External Quality Assessment Service study"],"prefix":"10.1111","volume":"85","author":[{"given":"P.J.","family":"Monaghan","sequence":"first","affiliation":[{"name":"The Christie Pathology Partnership The Christie NHS Foundation Trust Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kyriacou","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The Christie NHS Foundation Trust Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Sturgeon","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine UK NEQAS [Edinburgh] Royal Infirmary of Edinburgh Edinburgh UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Davies","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences & Medical and Human Sciences University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.J.","family":"Trainer","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The Christie NHS Foundation Trust Manchester UK"},{"name":"Centre for Endocrinology & Diabetes Institute of Human Development University of Manchester Manchester Academic Health Science Centre Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"White","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences & Medical and Human Sciences University of Manchester Manchester UK"},{"name":"Centre for Endocrinology & Diabetes Institute of Human Development University of Manchester Manchester Academic Health Science Centre Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.E.","family":"Higham","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The Christie NHS Foundation Trust Manchester UK"},{"name":"Centre for Endocrinology & Diabetes Institute of Human Development University of Manchester Manchester Academic Health Science Centre Manchester 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