{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:17:55Z","timestamp":1790248675051,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,7,2]],"date-time":"2017-07-02T00:00:00Z","timestamp":1498953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008018","name":"Victorian Cancer Agency","doi-asserted-by":"publisher","award":["EGCS 13027"],"award-info":[{"award-number":["EGCS 13027"]}],"id":[{"id":"10.13039\/100008018","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Advances in cancer treatment have led to improved long\u2010term survival after childhood cancer, but often at a price of impaired future fertility. Fertility preservation (<jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content>) in male children and early adolescents poses unique challenges as efficacy is unproven.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To describe characteristics of testicular tissue cryopreservation (<jats:styled-content style=\"fixed-case\">TTCP<\/jats:styled-content>) specimens taken from paediatric and adolescent patients, stratified by age, and prior chemotherapy, if any, and to demonstrate evidence for germ cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Retrospective review of gonadal biopsies and clinical records of patients consented into the Royal Children's Hospital <jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content> programme between 1987 and 2015. Tissue was sliced into blocks, with one section sent for histopathology prior to cryopreservation. In boys \u226512\u00a0years where spermatogenesis could be expected, a portion of tissue was disaggregated completely to look for mature sperm and if found, additional tissue was dissected and the resulting suspension frozen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Testicular tissue cryopreservation specimens in 44 males (0.3\u201016.8\u00a0years) provided an average of 7.8 slices per patient. All the specimens were taken at the same time as another necessary surgical procedure, under one general anaesthesic. There was only one complication of scrotal wound dehiscence. Seven of the forty\u2010four (15.9%) patients had chemotherapy prior to testicular biopsy, while the rest were chemotherapy na\u00efve. Five of these were prepubertal, and two were pubertal patients. Eleven subjects had tissue dissected with mature sperm found in eight. Of these eight patients where sperm were found, all were pubertal with testicular size of more than 10\u00a0mL and showing histological evidence of spermatogenesis. No histologic specimen demonstrated any malignant cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Testicular tissue cryopreservation can be performed in young patients without delay, preferably prior to cancer treatment. As testicular tissue contains germ cells from which haploid spermatozoa are ultimately derived, future technologies may allow their utilization for fertility in humans. This may be the only hope for biological offspring in some patients undergoing fertility compromising treatment. Retrieval of mature sperm from some pubertal patients, however, offers realistic hope to these patients of future fertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.13377","type":"journal-article","created":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T15:52:46Z","timestamp":1494863566000},"page":"279-285","source":"Crossref","is-referenced-by-count":48,"title":["A short report on current fertility preservation strategies for boys"],"prefix":"10.1111","volume":"87","author":[{"given":"Wei Li Cindy","family":"Ho","sequence":"first","affiliation":[{"name":"Royal Children's Hospital  Melbourne Vic. Australia"},{"name":"Khoo Teck Puat\u2010National University Children's Medical Institute National University Health System  Singapore"},{"name":"Murdoch Children's Research Institute  Parkville Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold","family":"Bourne","sequence":"additional","affiliation":[{"name":"Melbourne IVF  East Melbourne Vic. Australia"},{"name":"Reproductive Services Royal Women's Hospital  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debra","family":"Gook","sequence":"additional","affiliation":[{"name":"Melbourne IVF  East Melbourne Vic. Australia"},{"name":"Reproductive Services Royal Women's Hospital  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Clarke","sequence":"additional","affiliation":[{"name":"Andrology Unit Laboratory Services Department Royal Children's and Royal Women's Hospital  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Kemertzis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology Royal Women's Hospital University of Melbourne  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate","family":"Stern","sequence":"additional","affiliation":[{"name":"Melbourne IVF  East Melbourne Vic. Australia"},{"name":"Reproductive Services Royal Women's Hospital  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franca","family":"Agresta","sequence":"additional","affiliation":[{"name":"Melbourne IVF  East Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Heloury","sequence":"additional","affiliation":[{"name":"Royal Children's Hospital  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Clark","sequence":"additional","affiliation":[{"name":"Monash University  Clayton Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Orme","sequence":"additional","affiliation":[{"name":"Royal Children's Hospital  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin","family":"Jayasinghe","sequence":"additional","affiliation":[{"name":"Royal Children's Hospital  Melbourne Vic. Australia"},{"name":"Department of Obstetrics &amp; Gynaecology Royal Women's Hospital University of Melbourne  Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret R.","family":"Zacharin","sequence":"additional","affiliation":[{"name":"Royal Children's Hospital  Melbourne Vic. Australia"},{"name":"Murdoch Children's Research Institute  Parkville Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the Paediatric & Adolescent Fertility Preservation Task Force,\u00a0Melbourne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.21.1425"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181f87c4b"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000067835"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/det039"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dem062"},{"key":"e_1_2_8_7_1","unstructured":"Assisted Reproductive Treatment ActNo. 76 of 2008.2008http:\/\/www.legislation.vic.gov.au\/Domino\/Web_Notes\/LDMS\/PubStatbook.nsf\/edfb620cf7503d1aca256da4001b08af\/3ADFC9FBA2C0F526CA25751C0020E494\/$FILE\/08-076a.pdf. 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