{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T23:58:15Z","timestamp":1768435095982,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,8,18]],"date-time":"2017-08-18T00:00:00Z","timestamp":1503014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/1024291\/1"],"award-info":[{"award-number":["BB\/1024291\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000837","name":"University of Nottingham","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000771","name":"Prostate Cancer UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000771","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","award":["NIHR\u2010CS\u2010012\u2010009"],"award-info":[{"award-number":["NIHR\u2010CS\u2010012\u2010009"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"Summary<\/jats:title>Objective<\/jats:title>Women with a prior history of polycystic ovary syndrome (PCOS<\/jats:styled-content>) have an increased risk of endometrial cancer (EC<\/jats:styled-content>).<\/jats:p><\/jats:sec>Aim<\/jats:title>To investigate whether the endometrium of women withPCOS<\/jats:styled-content>possesses gene expression changes similar to those found inEC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Design and Methods<\/jats:title>Patients withEC<\/jats:styled-content>,PCOS<\/jats:styled-content>and control women unaffected by eitherPCOS<\/jats:styled-content>orEC<\/jats:styled-content>were recruited into a cross\u2010sectional study at the Nottingham University Hospital,UK<\/jats:styled-content>. ForRNA<\/jats:styled-content>sequencing, representative individual endometrial biopsies were obtained from women withEC<\/jats:styled-content>,PCOS<\/jats:styled-content>and a woman unaffected byPCOS<\/jats:styled-content>orEC<\/jats:styled-content>. Expression of a subset of differentially expressed genes identified byRNA<\/jats:styled-content>sequencing, includingNAD<\/jats:styled-content>(P)H quinone dehydrogenase 1 (NQO<\/jats:styled-content>1<\/jats:italic>), was validated by quantitative reverse transcriptasePCR<\/jats:styled-content>validation (n\u00a0=\u00a076) and in the cancer genome atlasUCEC<\/jats:styled-content>(uterine corpus endometrioid carcinoma)RNA<\/jats:styled-content>sequencing data set (n\u00a0=\u00a0381). The expression ofNQO<\/jats:styled-content>1 was validated by immunohistochemistry inEC<\/jats:styled-content>samples from a separate cohort (n\u00a0=\u00a091) comprised of consecutive patients who underwent hysterectomy at St Mary's Hospital, Manchester, between 2011 and 2013. A further 6 postmenopausal women with histologically normal endometrium who underwent hysterectomy for genital prolapse were also included. Informed consent and local ethics approval were obtained for the study.<\/jats:p><\/jats:sec>Results<\/jats:title>We show for the first thatNQO<\/jats:styled-content>1<\/jats:italic>expression is significantly increased in the endometrium of women withPCOS<\/jats:styled-content>andEC<\/jats:styled-content>. Immunohistochemistry confirms significantly increasedNQO<\/jats:styled-content>1 protein expression inEC<\/jats:styled-content>relative to nonmalignant endometrial tissue (P\u00a0<\/jats:italic><\u00a0.0001).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The results obtained here support a previously unrecognized molecular link betweenPCOS<\/jats:styled-content>andEC<\/jats:styled-content>involvingNQO<\/jats:styled-content>1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.13436","type":"journal-article","created":{"date-parts":[[2017,7,27]],"date-time":"2017-07-27T08:26:38Z","timestamp":1501143998000},"page":"557-565","source":"Crossref","is-referenced-by-count":14,"title":["Expression ofNAD<\/scp>(P)H quinone dehydrogenase 1 (NQO<\/scp>1) is increased in the endometrium of women with endometrial cancer and women with polycystic ovary syndrome"],"prefix":"10.1111","volume":"87","author":[{"given":"William","family":"Atiomo","sequence":"first","affiliation":[{"name":"Faculty of Medicine and 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Cancer Sciences Medicine and Health University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Kitson","sequence":"additional","affiliation":[{"name":"Faculty of Biology Division of Molecular & Clinical Cancer Sciences Medicine and Health University of Manchester Manchester UK"},{"name":"Department of Obstetrics and Gynaecology Central Manchester University Hospitals NHS Foundation Trust Manchester Academic Health Science Centre Manchester UK"},{"name":"Manchester School of Pharmacy University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanitha N.","family":"Sivalingam","sequence":"additional","affiliation":[{"name":"Faculty of Biology Division of Molecular & Clinical Cancer Sciences Medicine and Health University of Manchester Manchester UK"},{"name":"Department of Obstetrics and Gynaecology Central Manchester University Hospitals NHS Foundation Trust Manchester Academic Health Science Centre Manchester UK"},{"name":"Manchester School of Pharmacy University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian J.","family":"Stratford","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health Division of Pharmacy and Optometry School of Health Sciences University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catrin S.","family":"Rutland","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Sciences School of Veterinary Medicine and Science University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny L.","family":"Persson","sequence":"additional","affiliation":[{"name":"Clinical Research Center Lund University Malm\u00f6 Sweden"},{"name":"Department of Molecular Bology Ume\u00e5 University Ume\u00e5 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