{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T21:58:29Z","timestamp":1787522309105,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,5,15]],"date-time":"2019-05-15T00:00:00Z","timestamp":1557878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Polycystic ovarian syndrome (PCOS) is a heterogeneous endocrine disorder associated with mitochondrial dysfunction and insulin resistance (IR). MOTS\u2010c, a mitochondrial peptide, promotes insulin sensitivity (IS) through activating AKT and AMPK\u2010dependent pathways. The current study was designed to examine the response of MOTS\u2010c to lipids (intralipid) followed by insulin in PCOS and healthy subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>All subjects underwent 5\u2010hour intralipid\/saline infusion with a hyperinsulinemic\u2010euglycaemic clamp in the final 2\u00a0hours. Plasma samples were collected to measure circulating MOTS\u2010c using a commercial ELISA kit. Subsequently, this was repeated following an eight\u2010week exercise intervention.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Intralipid significantly increased plasma MOTS\u2010c both in controls and PCOS subjects, whilst the insulin infusion blunted the intralipid\u2010induced response seen for both lipids and MOT\u2010c.<\/jats:p><jats:p>Intralipid elevated plasma MOTS\u2010c to 232\u00a0\u00b1\u00a0124% of basal in control (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and to 349\u00a0\u00b1\u00a0206% of basal in PCOS (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) subjects. Administration of insulin suppressed intralipid\u2010induced MOTS\u2010c from 232\u00a0\u00b1\u00a0124% to 165\u00a0\u00b1\u00a097% (NS) in control and from 349\u00a0\u00b1\u00a0206% to 183\u00a0\u00b1\u00a0177% (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) in PCOS subjects, respectively. Following exercise, intralipid elevated plasma MOTS\u2010c to 305\u00a0\u00b1\u00a0153% of basal in control (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and to 215\u00a0\u00b1\u00a0103% of basal in PCOS (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) subjects; insulin suppressed intralipid\u2010induced MOTS\u2010c only in controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, this is the first study to show increased lipid enhanced circulating MOTS\u2010c whilst insulin attenuated the MOTS\u2010c response in human. Further, eight weeks of moderate exercise training did not show any changes in circulating MOTS\u2010c levels in healthy controls and in women with PCOS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.14007","type":"journal-article","created":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T02:05:00Z","timestamp":1557281100000},"page":"278-287","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Lipids and insulin regulate mitochondrial\u2010derived peptide (MOTS\u2010c) in PCOS and healthy subjects"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9765-9818","authenticated-orcid":false,"given":"Manjunath","family":"Ramanjaneya","sequence":"first","affiliation":[{"name":"Qatar Metabolic Institute, Interim Translational Research Institute, Academic Health System Hamad Medical Corporation Doha Qatar"},{"name":"Department of Medicine Hamad Medical Corporation Doha 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