{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:33:23Z","timestamp":1792172003812,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T00:00:00Z","timestamp":1570320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005378","name":"Hacettepe \u00dcniversitesi","doi-asserted-by":"publisher","award":["TSA\u20102018\u201017528"],"award-info":[{"award-number":["TSA\u20102018\u201017528"]}],"id":[{"id":"10.13039\/501100005378","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Polycystic ovary syndrome (PCOS) is associated with an increased cardiometabolic risk that might not necessarily translate into adverse cardiovascular outcome later in life. Recently, alterations in gut microbial composition have been reported in the syndrome. Microbiota\u2010dependent metabolite trimethylamine N\u2010oxide (TMAO) and its precursors are closely linked with development of atherosclerotic cardiovascular disease, independently of traditional risk factors. We aimed to assess whether TMAO and its precursors are altered in PCOS and to determine potential impact of treatment on these metabolites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients<\/jats:title><jats:p>Twenty\u2010seven overweight\/obese patients with PCOS and 25 age\u2010 and BMI\u2010matched healthy control women.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurements<\/jats:title><jats:p>At baseline, fasting serum TMAO and its precursors were measured after a 3\u2010day standardized diet. Patients received 3\u2010month OC therapy along with general dietary advice after which all measurements were repeated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients had higher total testosterone (T) and free androgen index (FAI) whereas whole\u2010body fat mass, fasting plasma glucose, insulin and lipids were similar between the groups. PCOS group showed significantly higher serum levels of TMAO and its precursors; choline, betaine and carnitine. TMAO and choline showed correlations with T. After 3\u00a0months of OC use, TMAO and its precursors significantly decreased along with reductions in BMI, T and FAI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study reports for the first time that TMAO and its precursors are elevated in PCOS which might contribute to increased cardiometabolic risk of the syndrome and that short\u2010term OC use along with lifestyle intervention is associated with reduction of these microbiome\u2010dependent metabolites.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.14101","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T06:01:11Z","timestamp":1569477671000},"page":"810-815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Circulating gut microbiota metabolite trimethylamine N\u2010oxide and oral contraceptive use in polycystic ovary syndrome"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1617-5134","authenticated-orcid":false,"given":"Nesrin Damla","family":"Eyupoglu","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Hacettepe University School of Medicine Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9574-8032","authenticated-orcid":false,"given":"Ezgi","family":"Caliskan Guzelce","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Hacettepe University School of Medicine Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aylin","family":"Acikgoz","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics Hacettepe University School of Health Sciences Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esra","family":"Uyanik","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Hacettepe University School of Medicine Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bodil","family":"Bj\u00f8rndal","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf K.","family":"Berge","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences University of Bergen Bergen Norway"},{"name":"Department of Heart Disease Haukeland University Hospital Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asbj\u00f8rn","family":"Svardal","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1797-7662","authenticated-orcid":false,"given":"Bulent Okan","family":"Yildiz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Hacettepe University School of Medicine Ankara Turkey"},{"name":"Division of Endocrinology and Metabolism Hacettepe University School of Medicine Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.57"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.13795"},{"key":"e_1_2_8_4_1","first-page":"324","article-title":"Dysbiosis of gut microbiota associated with clinical parameters in polycystic ovary syndrome","volume":"8","author":"Liu R","year":"2017","journal-title":"Front Microbiol"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168390"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2017-02153"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2017-02799"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146509"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1223813"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.149"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09922"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1569330"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqy116"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.004947"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehx342"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13307"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.10.004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2009.10.016"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2017.1295440"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12328"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.08.024"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.06.096"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.115.121269"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/01443615.2016.1264375"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/den109"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00168"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-3196"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.05.077"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.22271"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/met.2015.0120"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11010179"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26745"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0161425"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc17-2108"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcen.14101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.14101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cen.14101","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.14101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T22:27:57Z","timestamp":1694212077000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cen.14101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,6]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1111\/cen.14101"],"URL":"https:\/\/doi.org\/10.1111\/cen.14101","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,6]]},"assertion":[{"value":"2019-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}