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The inheritance pattern of the genes was analyzed in family trios or quartets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 205 patients, 83 patients carried at least one variant in 19 genes related to TD, and 59 of those 83 patients harbored more than two variants in distinct candidate genes for CH. Biallelic or <jats:italic>de novo<\/jats:italic> variants in the genes related to TD in Chinese patients are rare. We also found nine probands carried only one heterozygous variant in the genes related to TD that were inherited from a euthyroid either paternal or maternal parent. These findings did not support the monogenic inheritance pattern of the genes related to TD in CH patients. Notably, in family trio or quartet analysis, of 36 patients carrying more than two variants in distinct genes, 24 patients carried these variants inherited from both their parents, which indicated that the oligogenic inheritance pattern of the genes related to TD should be considered in CH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study expanded the variant spectrum of the genes related to TD in Chinese CH patients. It is rare that CH in Chinese patients could be explained by monogenic germline variants in genes related to TD. The hypothesis of an oligogenic origin of the CH should be considered.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.14577","type":"journal-article","created":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T04:29:19Z","timestamp":1628569759000},"page":"617-626","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The mutation screening in candidate genes related to thyroid dysgenesis by targeted next\u2010generation sequencing panel in the Chinese congenital hypothyroidism"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4485-1398","authenticated-orcid":false,"given":"Rui\u2010Jia","family":"Zhang","sequence":"first","affiliation":[{"name":"The Core Laboratory in Medical Center of Clinical Research, State Key Laboratory of Medical Genomics, Department of Molecular Diagnostics &amp; Endocrinology, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine 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