{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:23:59Z","timestamp":1790821439502,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,9,5]],"date-time":"2021-09-05T00:00:00Z","timestamp":1630800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Measurements of plasma free metanephrines (MNs), including MN and normetanephrine, provide high sensitivity and specificity for the diagnosis of pheochromocytoma and paraganglioma (PPGL). 3\u2010Methoxytyramine (3\u2010MT) and chromogranin A (CgA) may allow the detection of dopamine\u2010producing or biochemically silent PPGL. The aim of this study was to evaluate whether measurements of plasma 3\u2010MT or CgA as a supplement of plasma MNs offer a better diagnostic strategy for initial testing of PPGL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Design<\/jats:title><jats:p>We enroled 125 patients who underwent surgery from 2015 to 2016 for our study and identified 33 patients with PPGL and 92 patients with non\u2010PPGL masses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurement<\/jats:title><jats:p>The levels of plasma free MNs and 3\u2010MT were measured for all 125 patients using liquid chromatography\u2013tandem mass spectrometry. Plasma CgA concentrations were determined using a radioimmunoassay. To evaluate the diagnostic performance of plasma free MNs, 3\u2010MT and CgA, sensitivity and specificity were determined, and receiver operating characteristic curves were constructed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that combining 3\u2010MT and MNs increased the diagnostic sensitivity from 93.9% (95% confidence interval [CI]: 78.4%\u201398.9%) to 97.0% (95% CI: 82.5%\u201399.8%). In contrast, addition of plasma CgA test reduced the diagnostic specificity significantly from 91.3% (95% CI: 83.1%\u201395.9%) to 75.0% (95% CI: 64.7%\u201383.2%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Here, we demonstrated that 3\u2010MT represents a valuable supplementary test to plasma MNs, which can further enhance the sensitivity of the assay, while plasma CgA added no additional diagnostic value to MNs due to the lowered diagnostic specificity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.14585","type":"journal-article","created":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T05:25:35Z","timestamp":1630905935000},"page":"132-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Addition of 3\u2010methoxytyramine or chromogranin A to plasma free metanephrines as the initial test for pheochromocytoma and paraganglioma: Which is the best diagnostic strategy"],"prefix":"10.1111","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2939-5897","authenticated-orcid":false,"given":"Lu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Endocrinology Peking University First Hospital  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1708-0580","authenticated-orcid":false,"given":"Wenchun","family":"Xie","sequence":"additional","affiliation":[{"name":"Laboratory of Interdisciplinary Research Institute of Biophysics, Chinese Academy of Sciences  Beijing PR China"},{"name":"Department of Clinical Chemistry Guang Dong Bio\u2010healtech Advanced Co., Ltd.  Foshan PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijing","family":"Song","sequence":"additional","affiliation":[{"name":"Laboratory of Interdisciplinary Research Institute of Biophysics, Chinese Academy of Sciences  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Peking University First Hospital  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuesong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Peking University First Hospital  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Peking University First Hospital  Beijing PR 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