{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:17:58Z","timestamp":1791505078518,"version":"4.3.4"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T00:00:00Z","timestamp":1636156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100016275","name":"Seoul National University Bundang Hospital","doi-asserted-by":"publisher","award":["06\u20102020\u2010143"],"award-info":[{"award-number":["06\u20102020\u2010143"]}],"id":[{"id":"10.13039\/100016275","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Primary aldosteronism (PA) shows histological heterogeneity and clinical variability, including the coexistence of hypercortisolemia. Immunohistochemical analyses of steroidogenic enzymes in adrenal tissues have provided new insights into the pathogenesis of PA. However, a comprehensive analysis of the association between enzyme expression and clinical characteristics of PA has rarely been conducted. We aimed to investigate the correlation between clinical characteristics and steroidogenic enzyme expression in PA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>A retrospective case\u2010control study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients<\/jats:title><jats:p>Consecutive patients who underwent unilateral adrenalectomy for PA (<jats:italic>n<\/jats:italic>\u2009=\u2009180). Patients with adrenal Cushing's syndrome (CS) (<jats:italic>n<\/jats:italic>\u2009=\u200929) and nonfunctioning adenoma (<jats:italic>n<\/jats:italic>\u2009=\u20096) as comparator groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurements<\/jats:title><jats:p>A tissue microarray of adrenal adenomas was constructed and immunostained for CYP11B1, CYP11B2 and CYP17A1. The expression of the three enzymes was compared between PA and other adrenal diseases and between PA with and without mild autonomous cortisol excess (MACE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Adrenal adenomas in PA showed lower CYP11B1, higher CYP11B2 and lower CYP17A1 expression than those in adrenal CS (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). Nonfunctioning adenomas showed low expression of the three enzymes. PA with MACE showed higher CYP11B1 expression than PA without MACE. CYP11B1 expression was positively correlated with the severity of hypercortisolemia, and CYP11B2 was positively correlated with that of hyperaldosteronism. The expression of CYP11B1 and CYP11B2 had a negative correlation. Patients with absent clinical improvement after adrenalectomy had lower CYP11B2 expression than those with complete success.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Variable expression of steroidogenic enzymes in adrenal adenoma underlies the clinical heterogeneity of PA and is associated with treatment outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.14628","type":"journal-article","created":{"date-parts":[[2021,11,7]],"date-time":"2021-11-07T06:07:15Z","timestamp":1636265235000},"page":"30-39","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Expression of CYP11B1 and CYP11B2 in adrenal adenoma correlates with clinical characteristics of primary aldosteronism"],"prefix":"10.1111","volume":"96","author":[{"given":"Chang Ho","family":"Ahn","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Internal Medicine Seoul National University Bundang Hospital Seongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Young","family":"Na","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Pathology Seoul National University Bundang Hospital Seongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So Yeon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Pathology Seoul National University Bundang Hospital Seongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeong Won","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Surgery Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Surgery Seoul National University Bundang Hospital Seongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u2010Jin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Surgery Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Surgery Seoul National University Hospital Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"June Young","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Surgery Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Surgery Seoul National University Bundang Hospital Seongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu Eun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Surgery Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Surgery Seoul National University Hospital Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Wan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Internal Medicine Seoul Metropolitan Government Seoul National University Boramae Medical Center Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong Cheon","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Translational Medicine Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Integrated Major in Innovative Medical Science Seoul National University Graduate School Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1932-0234","authenticated-orcid":false,"given":"Jung Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University College of Medicine Seoul Republic of Korea"},{"name":"Department of Internal Medicine Seoul National University Hospital Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60834-X"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.93136"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2019-00299"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgab099"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.15328"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.11.022"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2014.05.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.11.014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2021.101"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgaa484"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgaa379"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2020.901"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(17)30135-3"},{"issue":"8","key":"e_1_2_9_15_1","first-page":"716","article-title":"Estrogen receptor analyses. Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies","volume":"109","author":"McCarty KS","year":"1985","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-2010"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1470795"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909471106"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.07.016"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-021476"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.04.022"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.02944"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.14628","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cen.14628","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.14628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T14:32:39Z","timestamp":1693233159000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cen.14628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,6]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1111\/cen.14628"],"URL":"https:\/\/doi.org\/10.1111\/cen.14628","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,6]]},"assertion":[{"value":"2021-08-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}