{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T23:05:25Z","timestamp":1771023925176,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,15]],"date-time":"2022-05-15T00:00:00Z","timestamp":1652572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Patients with congenital adrenal hyperplasia (CAH) in developing countries have limited access to appropriate laboratory facilities for diagnosis and follow\u2010up. The aim of this study is to evaluate steroid measurement in hair as a diagnostic tool to identify and monitor CAH in these patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>A method was developed to measure steroids in hair, the stability of steroids in hair was assessed, and the concentration range in healthy volunteers was determined. Hair samples of patients, before and after starting therapy, were transported at ambient temperature to The Netherlands for analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients<\/jats:title><jats:p>Twenty\u2010two Indonesian CAH patients and 84 healthy volunteers participated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurements<\/jats:title><jats:p>Cortisol, 17\u2010hydroxyprogesterone (17OHP), androstenedione, and testosterone in hair were measured by liquid chromatography with tandem mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Steroids in hair could be measured and remained stable (&lt;4.9% deviation) for at least 3 weeks at 4\u00b0C and 30\u00b0C. In each of the untreated patients, hair concentrations of 17OHP (9.43\u20131135\u2009pmol\/g), androstenedione (36.1\u2013432\u2009pmol\/g), and testosterone (2.85\u201369.2 pmol\/g) were all above the upper limit of the corresponding range in healthy volunteers; 5.5\u2009pmol\/g, 13\u2009pmol\/g, and 1.8\u2009pmol\/g, respectively. After starting glucocorticoid treatment, the steroid concentrations in the hair of CAH patients decreased significantly for androstenedione (73%) and testosterone (59%) after 6 months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CAH could be confirmed in Indonesian patients based on the concentration of 17OHP, androstenedione, and testosterone in hair, and a treatment effect was observed. These findings open up opportunities to diagnose and\/or monitor CAH in developing countries with a simple noninvasive technique.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.14754","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T04:14:25Z","timestamp":1651810465000},"page":"41-48","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Measuring steroids in hair opens up possibilities to identify congenital adrenal hyperplasia in developing countries"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5358-5303","authenticated-orcid":false,"given":"Selma","family":"Waaijers","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Radboud University Medical Center, Radboud Institute for Molecular Life Sciences Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agustini","family":"Utari","sequence":"additional","affiliation":[{"name":"Division of Human Genetics Center for Biomedical Research (CEBIOR), Faculty of Medicine Diponegoro University Semarang Indonesia"},{"name":"Division of Pediatric Endocrinology, Department of Pediatrics Faculty of Medicine Diponegoro University Semarang Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rick H. A.","family":"van der Doelen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Radboud University Medical Center, Radboud Institute for Molecular Life Sciences Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sultana M. H.","family":"Faradz","sequence":"additional","affiliation":[{"name":"Division of Human Genetics Center for Biomedical Research (CEBIOR), Faculty of Medicine Diponegoro University Semarang Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renate","family":"Hensen\u2010Lodewijk","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Radboud University Medical Center, Radboud Institute for Molecular Life Sciences Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre J.","family":"Olthaar","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Radboud University Medical Center, Radboud Institute for Molecular Life Sciences Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Geutjes","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Radboud University Medical Center, Radboud 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