{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T05:25:57Z","timestamp":1782969957552,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective and Design<\/jats:title><jats:p>Macroprolactinemia may influence the interpretation of serum prolactin levels\u2014a recognised phenomenon since 1981. The degree of macroprolactinaemia over time is less well described. We determined how macroprolactin status (based on polyethylene glycol (PEG) precipitation) varied by analysing serial measurements in hyperprolactinaemic individuals over a period of 9 years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Measurements<\/jats:title><jats:p>Results from 1810 individuals were included. All serum total prolactin results (measured using Roche Cobas 8000 analyser) were extracted from the laboratory information system for the period 1 January 2012 to 1 April 2021, along with relevant patient demographic\/test data. Samples with a macroprolactin screening test performed (on samples with prolactin\u2009&gt;\u2009700\u2009miu\/L) were included in the main analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>During the study period, 2782 macroprolactin checks were performed (12.5% of all prolactin tests) in 1810 individuals (599 males\/2183 females, median\u2010age: 35,\u00a0interquartile\u00a0range: 25\u201347, range: 16\u201393 years). Multiple macroprolactin checks were carried out on 465 patients (1437 measurements) with 94 patients (141 measurements) screening positive (&lt;60% recovery). Only 19 patients (18 female) had at least one result above and one below the 60% screening cut\u2010off, with 10 of these patients having results close to the 60% cut\u2010off; in 9 patients, results were clearly different between repeat samples. In seven cases, the adjusted monomeric prolactin showed a potentially clinically significant difference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In this study, only 19\/465 patients appeared to change macroprolactin status based on a 60% PEG recovery cut\u2010off. The majority of these 19 patients were on antipsychotic\/antidepressant medication(s) or had a prolactinoma; in only 7\u00a0did monomeric prolactin change significantly. This suggests that once macroprolactin status has been determined, clinical decision making is rarely affected by repeating it.<\/jats:p><\/jats:sec>","DOI":"10.1111\/cen.15051","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T12:30:01Z","timestamp":1711542601000},"page":"450-458","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Macroprolactin over time: Is there any point in rechecking it in people with a persistently elevated serum prolactin?"],"prefix":"10.1111","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6878-0769","authenticated-orcid":false,"given":"Mark","family":"Livingston","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Black Country Pathology Services\u00a0 Walsall Manor Hospital Walsall UK"},{"name":"Faculty of Science and Engineering, School of Medicine and Clinical Practice The University of Wolverhampton Wolverhampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syeda F.","family":"Hashmi","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Black Country Pathology Services\u00a0 Walsall Manor Hospital Walsall UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudarshan","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry University Hospitals Birmingham NHS Foundation Trust Birmingham UK"},{"name":"Department of Clinical Biochemistry University Hospitals of North Midlands Stoke\u2010on\u2010Trent UK"},{"name":"Institute of Science and Technology Keele University Keele UK"},{"name":"Faculty of Health Sciences Staffordshire University Stoke\u2010on\u2010Trent UK"},{"name":"Department of Mechanical and Aerospace Engineering Brunel University London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Laing","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Royal Preston Hospital Preston UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9537-4050","authenticated-orcid":false,"given":"Adrian","family":"Heald","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Diabetes Salford Royal Hospital Salford UK"},{"name":"The School of Medicine and Manchester Academic Health Sciences Centre Manchester University Manchester 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elecsys prolactin II assay: reactivity with macroprolactin compared with eight commercial assays for prolactin and determination of monomeric prolactin by precipitation with polyethylene glycol","volume":"53","author":"Fahie\u2010Wilson M","year":"2007","journal-title":"Clin Lab"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2015-1283"},{"key":"e_1_2_10_16_1","first-page":"3","article-title":"Prolactin biology and laboratory measurement: an update on physiology and current analytical issues","volume":"39","author":"Saleem M","year":"2018","journal-title":"Clin Biochem Rev"},{"key":"e_1_2_10_17_1","first-page":"207","article-title":"Macroprolactinemia in women with hyperprolactinemia: a 10\u2010year follow\u2010up","volume":"34","author":"Radavelli\u2010Bagatini S","year":"2013","journal-title":"Neuro Endocrinol 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