{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:03:51Z","timestamp":1791644631577,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Advances in oncological and haematological treatments have markedly improved childhood survival, yet gonadotoxic therapies often compromise testicular function. Testicular tissue extraction (TTE) with cryopreservation is increasingly offered to prepubertal boys at high risk of infertility; however, data on long\u2010term endocrine and fertility outcomes remain limited. This study evaluated the long\u2010term endocrine and exocrine testicular function of males who underwent TTE prior to gonadotoxic therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design and Patients<\/jats:title>\n                    <jats:p>This was a retrospective, observational, single\u2010centre study including 50 males treated between 2009 and 2022 at Lille University Hospital (median age at TTE: 5.6 years; median age at last follow\u2010up: 14.6 years). Underlying conditions comprised malignant haematological disorders (52%), non\u2010malignant diseases (34%), and solid tumours (14%); 80% underwent allogeneic haematopoietic stem cell transplantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements<\/jats:title>\n                    <jats:p>Serial clinical assessments and biochemical markers of Leydig and Sertoli cell function (LH, FSH, testosterone, inhibin B, AMH) were recorded from puberty onset to young adulthood. Data were stratified by treatment intensity, pubertal timing, and disease type. Post\u2010pubertal semen analysis was performed in consenting individuals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All participants experienced spontaneous pubertal onset. Sertoli cell dysfunction, evidenced by elevated FSH and persistently low inhibin B, was detectable early in puberty and persisted into adulthood. At Tanner stage 5, 18% had elevated LH, 75% elevated FSH, and 89% low inhibin B; comparable rates were observed in adulthood. Radiotherapy was associated with higher gonadotropin levels at equivalent chemotherapy doses, and Sertoli cell impairment was more pronounced in malignant disease and peri\u2010pubertal treatment. Among 12 semen analyses, spermatozoa were detected in 42%, all in non\u2010malignant cases without radiotherapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Males undergoing TTE prior to gonadotoxic therapy frequently develop persistent Sertoli cell dysfunction, with limited fertility potential in many cases. These findings underscore the importance of systematic, long\u2010term andrological surveillance and personalised fertility counselling in this high\u2010risk population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/cen.70073","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T08:13:02Z","timestamp":1768378382000},"page":"590-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Andrological Outcomes in Boys Undergoing Testicular Tissue Extraction Prior to Gonadotoxic Treatment: Evolution During Peripuberty and Young Adulthood"],"prefix":"10.1111","volume":"104","author":[{"given":"Eulalie","family":"Martin","sequence":"first","affiliation":[{"name":"Department of Pediatric Endocrinology, DevGen, Reference Centre for Genital Development Abnormalities University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0443-8344","authenticated-orcid":false,"given":"Hamza","family":"Benderradji","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetology, and Metabolism University of Lille, CHU Lille Lille France"},{"name":"Department of Andrology, Urology and Renal Transplantation University of Lille, CHU Lille Lille France"},{"name":"University of Lille, CHU\u2010Lille, Inserm U1172, Lille Neuroscience &amp; Cognition Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Keller","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology\u2010Spermiology\u2010CECOS University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iva","family":"Gueorguieva","sequence":"additional","affiliation":[{"name":"Department of Pediatric Endocrinology, DevGen, Reference Centre for Genital Development Abnormalities University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Lefevre","sequence":"additional","affiliation":[{"name":"Department of Pediatric Endocrinology, DevGen, Reference Centre for Genital Development Abnormalities University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2301-702X","authenticated-orcid":false,"given":"Arthur Lauriot Dit","family":"Prevost","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bereng\u00e8re","family":"Ducrocq","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology\u2010Spermiology\u2010CECOS University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Marcelli","sequence":"additional","affiliation":[{"name":"Department of Andrology, Urology and Renal Transplantation University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Sophie","family":"Defachelles","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Oscar Lambret Cancer Center University of Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Pigny","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Hormonology, Metabolism\u2013Nutrition and Oncology Unit, CHU\u2010Lille University of Lille Lille France"},{"name":"University of Lille, Inserm, CHU Lille, U1277\u2010CANTHER\u2010Cancer\u2010Heterogeneity Plasticity and Resistance to Therapies Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedicte","family":"Bruno","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Leroy","sequence":"additional","affiliation":[{"name":"Department of Pediatric Endocrinology, DevGen, Reference Centre for Genital Development Abnormalities University of Lille, CHU Lille Lille France"},{"name":"Department of Andrology, Urology and Renal Transplantation University of Lille, CHU Lille Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)62038-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(17)30052-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41409-022-01755-x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705531"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pon.784"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpag.2016.04.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2016.02.2967"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.25078"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.854186"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2022.909000"},{"issue":"8","key":"e_1_2_9_12_1","article-title":"ESHRE Good Practice Recommendations on Fertility Preservation Involving Testicular Tissue Cryopreservation in Children Receiving Gonadotoxic Therapies","volume":"40","author":"Mitchell R. T.","year":"2025","journal-title":"Human Reproduction"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.28507"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-1207"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2016.08.005"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.204123"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70408-5"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgac218"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.54"},{"issue":"1","key":"e_1_2_9_20_1","first-page":"26","article-title":"Long\u2010Term Follow\u2010Up of Boys Who Have Undergone a Testicular Biopsy for Fertility Preservation","volume":"36","author":"Kanbar M.","year":"2021","journal-title":"Human Reproduction (Oxford, England)"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmaa050"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1292683"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.09.044"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav2914"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/903142"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1240339"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2022.05.013"}],"container-title":["Clinical Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.70073","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cen.70073","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cen.70073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:00:26Z","timestamp":1777431626000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cen.70073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,14]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/cen.70073"],"URL":"https:\/\/doi.org\/10.1111\/cen.70073","archive":["Portico"],"relation":{},"ISSN":["0300-0664","1365-2265"],"issn-type":[{"value":"0300-0664","type":"print"},{"value":"1365-2265","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,14]]},"assertion":[{"value":"2025-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}