{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:32:38Z","timestamp":1790199158025,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T00:00:00Z","timestamp":1369094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n The\n \n \n Ins2\n Akita<\/jats:sup>\n <\/jats:italic>\n <\/jats:styled-content>\n mouse has been reported to display retinal pathology degeneration associated with advanced diabetic retinopathy. In the present study, we monitored retinal changes in these mice to establish if this model displays clinical features associated with advanced diabetic retinopathy in human patients.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n \n \n Ins2\n Akita<\/jats:sup>\n <\/jats:italic>\n <\/jats:styled-content>\n mice (\n \n n<\/jats:styled-content>\n <\/jats:italic>\n \u2009=\u200955) on a\n C57Bl<\/jats:styled-content>\n \/\n 6J<\/jats:styled-content>\n background were monitored clinically from 9 to 25 weeks of age using a combination of scanning laser ophthalmoscopy, fluorescein angiography and optical coherence tomography. After clinical imaging, eyes were processed for immunostaining to examine microglial, astroglial and\n M<\/jats:styled-content>\n uller glial responses to hyperglycaemia. To complement our optical coherence tomography imaging, retinal morphology and thicknesses were examined in high\u2010quality semi\u2010thin sections.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n No retinal thinning or disruption of retinal architecture was observed by optical coherence tomography or resin histology in\n \n \n Ins2\n Akita<\/jats:sup>\n <\/jats:italic>\n <\/jats:styled-content>\n mice up to 6 months of age. In addition, no vascular changes were detected by fluorescein angiography or by scanning laser ophthalmoscopy. With the exception of microglial activation, reduced glial fibrillary acid protein expression in astrocytes and an increase in glial fibrillary acid protein expression by\n M<\/jats:styled-content>\n uller cells, no other changes were observed in the\n \n \n Ins2\n Akita<\/jats:sup>\n <\/jats:italic>\n <\/jats:styled-content>\n retina.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our results indicate that the classical clinical correlates of human diabetic retinopathy are absent in\n \n \n Ins2\n Akita<\/jats:sup>\n <\/jats:italic>\n <\/jats:styled-content>\n mice up to 6 months of age suggesting that either the histopathological processes underlying the development of diabetic retinopathy in this model require longer than 5 months of hyperglycaemia to result in disruption of retinal architecture or that advanced diabetic retinopathy is not a feature of the\n \n \n Ins2\n Akita<\/jats:sup>\n <\/jats:italic>\n <\/jats:styled-content>\n diabetic mouse.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ceo.12084","type":"journal-article","created":{"date-parts":[[2013,2,21]],"date-time":"2013-02-21T23:14:29Z","timestamp":1361488469000},"page":"582-592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Absence of clinical correlates of diabetic retinopathy in the\n \n \n Ins2\n Akita<\/sup>\n <\/i>\n <\/scp>\n retina"],"prefix":"10.1111","volume":"41","author":[{"given":"Samuel","family":"McLenachan","sequence":"first","affiliation":[{"name":"Centre for Ophthalmology and Visual Science The University of Western Australia Perth Western Australia Australia"},{"name":"Department of Molecular Ophthalmology Lions Eye Institute Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology School of Biomedical Sciences Monash University Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul G","family":"McMenamin","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology School of Biomedical Sciences Monash University Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth P","family":"Rakoczy","sequence":"additional","affiliation":[{"name":"Centre for Ophthalmology and Visual Science The University of Western Australia Perth Western Australia Australia"},{"name":"Department of Molecular Ophthalmology Lions Eye Institute Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/09286580701396720"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.21.1.143"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.122.4.552"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1258\/la.2010.010090"},{"issue":"1","key":"e_1_2_5_6_1","first-page":"91","article-title":"Studies on the diabetogenic action of streptozotocin (NSC\u201037917)","volume":"29","author":"Rakieten N","year":"1963","journal-title":"Cancer Chemother Rep"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/108328"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.5.887"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-1340"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-1824"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.5.1559"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2008.08.024"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.6.3368"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035551"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-9662"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg232"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090883"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2010.551172"},{"key":"e_1_2_5_19_1","first-page":"3043","article-title":"Optimal methods for preparation and immunostaining of iris, ciliary body, and choroidal wholemounts","volume":"41","author":"McMenamin PG","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8601"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00873.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2010.05.010"},{"key":"e_1_2_5_23_1","first-page":"213","article-title":"Microglia in human retina: a heterogeneous population with distinct ontogenies","volume":"3","author":"Provis JM","year":"1996","journal-title":"Perspect Dev Neurobiol"},{"key":"e_1_2_5_24_1","first-page":"3561","article-title":"Altered expression of retinal occludin and glial fibrillary acidic protein in experimental diabetes. The Penn State Retina Research Group","volume":"41","author":"Barber AJ","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2425"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.visres.2006.11.017"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952523800173122"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(93)31513-7"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.86.7.725"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-3143"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-5041"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.visres.2010.08.024"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6293"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1376"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-5768"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2009.07.001"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-010-1900-z"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.3.445"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2011.09.030"}],"container-title":["Clinical & Experimental Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fceo.12084","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.12084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T08:15:38Z","timestamp":1696320938000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ceo.12084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,21]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1111\/ceo.12084"],"URL":"https:\/\/doi.org\/10.1111\/ceo.12084","archive":["Portico"],"relation":{},"ISSN":["1442-6404","1442-9071"],"issn-type":[{"value":"1442-6404","type":"print"},{"value":"1442-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,21]]},"assertion":[{"value":"2012-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}