{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:06:39Z","timestamp":1762430799049,"version":"3.37.3"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T00:00:00Z","timestamp":1390435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["626964; 508123"],"award-info":[{"award-number":["626964; 508123"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Microglial activation is a prominent feature throughout the optic pathway in experimental glaucoma. Pro\u2010inflammatory microglial activation may contribute to neurodegeneration through the release of pro\u2010inflammatory cytokines and other inflammatory mediators. Systemic administration of lipopolysaccharide stimulates microglia to produce pro\u2010inflammatory cytokines and chemoattractants. A preliminary investigation demonstrated pro\u2010inflammatory microglial activation throughout the optic pathway following systemic lipopolysaccharide challenge. The aim of the current work was to investigate whether microglial priming with lipopolysaccharide would exacerbate optic nerve injury in rats following experimental glaucoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult female <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>prague\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>awley rats were divided into lipopolysaccharide treatment (<jats:italic>n<\/jats:italic>\u2009=\u200915) and saline treatment groups (<jats:italic>n<\/jats:italic>\u2009=\u200915). Microglial priming was induced with a 2.5\u2010mg\/kg intraperitoneal injection of lipopolysaccharide; control animals received saline. Experimental glaucoma was induced 48\u2009h later in the right eyes of animals by laser photocoagulation of the trabecular meshwork. Animals were sacrificed 9 days after laser treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The estimated number of axons per optic nerve was 51\u2009327\u2009\u00b1\u20093868 (mean\u2009\u00b1\u2009standard error of the mean) in the lipopolysaccharide group and 54\u2009569\u2009\u00b1\u20096687 (mean\u2009\u00b1\u2009standard error of the mean) in the saline group. Optic nerve axon counts were not significantly different between lipopolysaccharide and saline groups (<jats:italic>P<\/jats:italic>\u2009=\u20090.67).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Systemic lipopolysaccharide challenge had no discernible effect on optic nerve injury in laser\u2010induced experimental glaucoma. These findings do not support the hypothesis that this model of experimental glaucoma involves inflammation and instead suggest that microglial activation may occur secondary to chronic neurodegeneration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.12289","type":"journal-article","created":{"date-parts":[[2013,12,24]],"date-time":"2013-12-24T11:22:49Z","timestamp":1387884169000},"page":"539-554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Immune priming and experimental glaucoma: the effect of prior systemic lipopolysaccharide challenge on tissue outcomes after optic nerve injury"],"prefix":"10.1111","volume":"42","author":[{"given":"Daniel S","family":"Narayan","sequence":"first","affiliation":[{"name":"Ophthalmic Research Laboratories South Australian Institute of Ophthalmology Hanson Institute Centre for Neurological Diseases  Adelaide South Australia Australia"},{"name":"Department of Ophthalmology 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