{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T02:52:20Z","timestamp":1763347940302},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,1,15]],"date-time":"2015-01-15T00:00:00Z","timestamp":1421280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Dr. Scientific Research Foundation of China","award":["No.20091115"],"award-info":[{"award-number":["No.20091115"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>To explore the anti\u2010angiogenesis mechanism of R<\/jats:styled-content>ofecoxib and determine whether R<\/jats:styled-content>ofecoxib can be a therapeutic agent for the prevention of retinal neovascularization using a model of retinopathy of prematurity (ROP<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>ROP<\/jats:styled-content> was induced by exposing mice to 75% oxygen from postnatal day 7 (P<\/jats:styled-content>7<\/jats:sub>) to P<\/jats:styled-content>12<\/jats:sub>, then to room air from P<\/jats:styled-content>12<\/jats:sub> to P<\/jats:styled-content>17<\/jats:sub>. Sixteen mice were in each of the three groups: untreated ROP<\/jats:styled-content> group as positive control, Rofecoxib\u2010treated ROP<\/jats:styled-content> group and the normal group (age\u2010matched mice maintained in room air from birth to P<\/jats:styled-content>17<\/jats:sub> as negative control). The localized expression of cyclooxygenase\u20102 (COX<\/jats:styled-content>\u20102) and vascular endothelial growth factor (VEGF<\/jats:styled-content>) protein and mRNA<\/jats:styled-content> in retinal blood vessels was assessed using immunohistochemistry, Western blot analysis and reverse transcription polymerase chain reaction.<\/jats:p><\/jats:sec>Results<\/jats:title>Mice in the R<\/jats:styled-content>ofecoxib\u2010treated group had a significantly reduced retinal neovascular tufts compared with those in the untreated ROP<\/jats:styled-content> group. COX<\/jats:styled-content>\u20102 and VEGF<\/jats:styled-content> protein and mRNA<\/jats:styled-content> expression levels were increased in the untreated ROP<\/jats:styled-content> group, compared with the normal group. R<\/jats:styled-content>ofecoxib decreased retinal angiogenesis by inhibiting COX<\/jats:styled-content>\u20102 and VEGF<\/jats:styled-content> expression. The expression levels of VEGF<\/jats:styled-content> and COX<\/jats:styled-content>\u20102 were positively correlated at mRNA<\/jats:styled-content> and protein levels.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>COX<\/jats:styled-content>\u20102 and VEGF<\/jats:styled-content> expressions were both involved in the regulation of angiogenesis and had the same cellular localization. Expression of COX<\/jats:styled-content>\u20102 correlated positively with VEGF<\/jats:styled-content> in retinal neovascularization. Rofecoxib attenuated retinal angiogenesis by inhibiting the expression of COX<\/jats:styled-content>\u20102 and VEGF mRNA<\/jats:styled-content> and protein.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.12473","type":"journal-article","created":{"date-parts":[[2014,12,5]],"date-time":"2014-12-05T23:53:14Z","timestamp":1417823594000},"page":"458-465","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Rofecoxib inhibits retinal neovascularization via down regulation of cyclooxygenase\u20102 and vascular endothelial growth factor expression"],"prefix":"10.1111","volume":"43","author":[{"given":"Ning\u2010ning","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology The First Affiliated Hospital of China Medical University Shenyang Liaoning 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