{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T11:54:54Z","timestamp":1764330894863},"reference-count":70,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,2,24]],"date-time":"2016-02-24T00:00:00Z","timestamp":1456272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To evaluate the regulation of blood supply in primary uveal melanomas through caveolin\u20101 (Cav\u20101)\/phosphoinositol\u20103 kinase (PI3K).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of Cav\u20101 and PI3K was analysed in 51 paraffin sections of metastatic (<jats:italic>n<\/jats:italic>\u2009=\u200930) and non\u2010metastastic uveal melanomas (<jats:italic>n<\/jats:italic>\u2009=\u200921). Two trained observers quantified Cav\u20101 and PI3K immunofluorescensce expression by determining intensity of staining and percentage of positive cells. The expression was correlated with known prognostic factors. Besides angiogenesis by means of endoglin expression, the normal vasculature (von Willebrand Factor expression) was evaluated semi\u2010quantitatively. Vasculogenic mimicry (VM) was analysed by CD31\/PAS staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All examined specimens expressed Cav\u20101 with a mean of 90.34% Cav\u20101 positive cells (range, 3.23\u2013100%). Metastatic disease was associated with a higher Cav\u20101 expression. The correlation of Cav\u20101 with well\u2010established prognostic factors showed a significant association between Cav\u20101 expression and largest tumour diameter (<jats:italic>P<\/jats:italic>\u2009=\u20090.022), tumour node metastasis classification (<jats:italic>P<\/jats:italic>\u2009=\u20090.008) and invasion of optic nerve head (<jats:italic>P<\/jats:italic>\u2009=\u20090.048). PI3K was expressed by all uveal melanomas with a mean of 87.28% cells showing PI3K expression. A higher level of PI3K was significantly associated with larger height (<jats:italic>P<\/jats:italic>\u2009=\u20090.042) and progressed tumour node metastasis stage (<jats:italic>P<\/jats:italic>\u2009=\u20090.016). The percentage of PI3K and Cav\u20101 positive cells were significantly associated (<jats:italic>P<\/jats:italic>\u2009=\u20090.034). For PI3K and Cav\u20101 expression a non\u2010significant association with VM was shown (<jats:italic>P<\/jats:italic>\u2009=\u20090.064 and <jats:italic>P<\/jats:italic>\u2009=\u20090.072, respectively). No correlation of PI3K or Cav\u20101 with angiogenesis or mature vasculature was seen (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cav\u20101 expression may be especially up\u2010regulated in larger uveal melanomas. As it was correlated with PI3K expression and VM in this series of uveal melanoma, Cav\u20101 might induce the formation of VM via the PI3K\u2010signalling cascade.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.12686","type":"journal-article","created":{"date-parts":[[2015,11,21]],"date-time":"2015-11-21T22:00:45Z","timestamp":1448143245000},"page":"400-409","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of caveolin\u20101 and phosphoinositol\u20103 kinase expression in primary uveal melanomas"],"prefix":"10.1111","volume":"44","author":[{"given":"Miriam","family":"Stenzel","sequence":"first","affiliation":[{"name":"Department of Ophthalmology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayseg\u00fcl","family":"Tura","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University of L\u00fcbeck  L\u00fcbeck 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