{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:28:09Z","timestamp":1740126489808,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T00:00:00Z","timestamp":1458345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To investigate the effects of commonly used intravitreal anti\u2010vascular endothelial growth factor (anti\u2010VEGF) antibodies on proliferation index and viability of mesenchymal stem cells derived from ciliary body and limbus (CB\u2010MSC and LMSC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CB\u2010MSCs and LMSCs were isolated from newborn rats' eyes, and they were expanded in medium by the explant method. Intravitreally used anti\u2010VEGF drugs, aflibercept, bevacizumab and ranibizumab were tested into the 16\u2010well plates, respectively, at four different concentrations. After keeping them for 48\u2009h, the proliferation indexes and viabilities of CB\u2010MSCs and LMSCs were compared separately by Real\u2010Time Cell Analyzer and Methylthiazoltetrazoli (MTT) test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Anti\u2010VEGFs used at 5\u2010times and 10\u2010times of the standard clinical dosage caused statistically significant negative effects on proliferation indexes of CB\u2010MSCs and LMSCs at the 24<jats:sup>th<\/jats:sup> hour compared to control group. Only the anti\u2010VEGF group that had 10\u2010times dosage of those used clinically had a statistically significant negative effect on the viabilties of CB\u2010MSCs and LMSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Administrations of high doses or repeated standard doses of intravitreal anti\u2010VEGF agents may affect the proliferation indexes and viabilities of CB\u2010MSCs and LMSCs adversely. These novel findings deserve further in vivo investigations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.12715","type":"journal-article","created":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T08:54:37Z","timestamp":1455008077000},"page":"587-596","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of commonly used intravitreal anti\u2010vascular endothelial growth factor drugs on mesenchymal stem cells derived from the limbus and ciliary body"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2950-8361","authenticated-orcid":false,"given":"Ugur","family":"Acar","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Hacettepe University, Faculty of Medicine  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damla","family":"Erginturk Acar","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Ministry of 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