{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:41:42Z","timestamp":1783690902399,"version":"3.55.0"},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,1,29]],"date-time":"2017-01-29T00:00:00Z","timestamp":1485648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Retinopathy of prematurity is one of the leading causes of childhood blindness worldwide, with vessel growth cessation and vessel loss in phase I followed by neovascularization in phase II. Ischaemia contributes to its pathogenesis, and lutein protects against ischaemia\u2010induced retinal damages. We aimed to investigate the effects of lutein on a murine model of oxygen\u2010induced retinopathy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mouse pups were exposed to 75% oxygen for 5\u00a0days and returned to room air for another 5\u00a0days. Vascular obliteration, neovascularization and blood vessel leakage were examined. Immunohistochemistry for glial cells and microglia were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with vehicle controls, mouse pups receiving lutein treatment displayed smaller central vaso\u2010obliterated area and reduced blood vessel leakage. No significant difference in neovascular area was found between lutein and vehicle controls. Lutein promoted endothelial tip cell formation and maintained the astrocytic template in the avascular area in oxygen\u2010induced retinopathy. No significant changes in M\u00fcller cell gliosis and microglial activation in the central avascular area were found in lutein\u2010treated pups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our observations indicated that lutein significantly promoted normal retinal vascular regrowth in the central avascular area, possibly through promoting endothelial tip cell formation and preserving astrocytic template. Our results indicated that lutein might be considered as a supplement for the treatment of proliferative retinopathy of prematurity because of its role in facilitating the revascularization of normal vasculature.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.12908","type":"journal-article","created":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T22:52:07Z","timestamp":1482360727000},"page":"529-538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Lutein facilitates physiological revascularization in a mouse model of retinopathy of prematurity"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8182-2983","authenticated-orcid":false,"given":"Zhongjie","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Li Ka Shing Faculty of Medicine University of Hong Kong  Hong Kong"},{"name":"Department of Ophthalmology Boston Children's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven S","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Boston Children's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel B","family":"Burnim","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Boston Children's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lois EH","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Boston Children's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy CY","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Li Ka Shing Faculty of Medicine University of Hong Kong  Hong Kong"},{"name":"Research Center of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine University of Hong Kong  Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,29]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"262","article-title":"Fibroblastic overgrowth of persistent tunica vasculosa lentis in infants born prematurely. 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