{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:40:04Z","timestamp":1782978004844,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,4,27]],"date-time":"2017-04-27T00:00:00Z","timestamp":1493251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Retinal ischaemia is a common feature shared by numerous eye diseases. Ischaemic insult leads to retinal dysfunction and neuronal death. <jats:italic>Lycium barbarum<\/jats:italic> polysaccharides are well known for eyesight preservation. We have previously reported the effect of <jats:italic>Lycium barbarum<\/jats:italic> polysaccharides on cell death, blood ocular barrier and oxidative stress within 24 h retinal ischaemia. This study focuses on retinal function and looks for ultrastructural and cellular correlates after a relatively long period of reperfusion for 7 days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two\u2010hour ischaemia was induced by intraluminal occlusion of the internal carotid artery. Either <jats:italic>Lycium barbarum<\/jats:italic> polysaccharides or phosphate\u2010buffered saline was orally pre\u2010administered daily for 7 days before ischaemia and continued for 1, 3 and 7 days after reperfusion. Electroretinogram was performed to evaluate visual function. Paraffin\u2010embedded retinal sections were prepared 7 days after reperfusion and utilized for histological and immunohistochemical analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ischaemia led to sustained inhibition of b\u2010wave amplitude and oscillatory potentials. <jats:italic>Lycium barbarum<\/jats:italic> polysaccharide\u2010treated mice exhibited greater b\u2010wave and oscillatory potential responses from days 1 to 7 after reperfusion. In addition, increased number of viable cells and calretinin\u2010positive cells, as well as enhanced immunoreactivity of protein kinase C alpha and attenuated glial fibrillary acidic protein expression, was noted in <jats:italic>Lycium barbarum<\/jats:italic> polysaccharide\u2010treated retina.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Daily consumption of <jats:italic>Lycium barbarum<\/jats:italic> polysaccharides effectively alleviated ischaemia\u2010induced retinal dysfunction as well as reduced correlated neuronal death and glial activation. This prolonged effect could last at least 7 days. It suggested that <jats:italic>Lycium barbarum<\/jats:italic> polysaccharides might serve as a neuroprotective agent in ischaemic retinopathies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.12950","type":"journal-article","created":{"date-parts":[[2017,3,28]],"date-time":"2017-03-28T02:39:25Z","timestamp":1490668765000},"page":"717-729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["<i>Lycium barbarum<\/i> polysaccharide extracts preserve retinal function and attenuate inner retinal neuronal damage in a mouse model of transient retinal ischaemia"],"prefix":"10.1111","volume":"45","author":[{"given":"Di","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, First Affiliated Hospital of Kunming Medical University Kunming Medical University  Kunming Yunnan China"},{"name":"Department of Ophthalmology, Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong 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