{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:45:51Z","timestamp":1761896751477,"version":"3.37.3"},"reference-count":14,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007811","name":"Sydney Medical School Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007811","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Exper Ophthalmology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Importance<\/jats:title><jats:p>Transient corneal endothelial changes are routinely noted on slit\u2010lamp examination immediately following selective laser trabeculoplasty (SLT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>To determine the mechanism of transient corneal endothelial changes observed following SLT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>University laboratory\u2010based observational study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Samples<\/jats:title><jats:p>Ten corneas from six human cadaveric donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Corneas were treated with SLT, direct laser or peroxide, or used as controls. Haematoxylin and eosin staining and immunolabelling for zonula occludens\u20101 (ZO\u20101) and beta\u2010catenin were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main Outcome Measures<\/jats:title><jats:p>Histological appearance; ZO\u20101 and beta\u2010catenin immunostaining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were no differences in histological features between SLT\u2010treated and control corneas. Corneas treated with SLT or peroxide showed reduced and less regular ZO\u20101 immunofluorescence along cell membranes compared with ZO\u20101 expression in controls. These changes were generalized across the endothelium. There was no effect on the ZO\u20101 immunostaining after direct laser. There was no difference in beta\u2010catenin immunostaining patterns between control, SLT and peroxide\u2010treated corneas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Relevance<\/jats:title><jats:p>Altered ZO\u20101 immunostaining may represent disassembly of tight junctions between corneal endothelial cells. The similarity of our findings between SLT\u2010treated and peroxide\u2010treated corneas suggests that both conditions trigger changes at the level of endothelial tight junctions, perhaps triggered by liberation of free radicals as previously proposed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ceo.13172","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T12:32:41Z","timestamp":1518611561000},"page":"730-737","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Investigation of corneal endothelial changes post selective laser trabeculoplasty"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2287-5513","authenticated-orcid":false,"given":"Kate E","family":"Leahy","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Westmead Hospital  Sydney New South Wales Australia"},{"name":"Sydney Medical School University of Sydney  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele C","family":"Madigan","sequence":"additional","affiliation":[{"name":"Save Sight Institute University of Sydney  Sydney New South Wales Australia"},{"name":"School of Optometry and Vision Science University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Sarris","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie L","family":"Watson","sequence":"additional","affiliation":[{"name":"Save Sight Institute University of Sydney  Sydney New South Wales Australia"},{"name":"School of Medical Sciences University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"McCluskey","sequence":"additional","affiliation":[{"name":"Save Sight Institute University of Sydney  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew JR","family":"White","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Westmead Hospital  Sydney New South Wales Australia"},{"name":"Save Sight Institute University of Sydney  Sydney New South Wales Australia"},{"name":"School of Optometry and Vision Science University of New South Wales  Sydney New South Wales Australia"},{"name":"Westmead Institute for Medical Research University of Sydney  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Australian Government Department of Human Services. 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Poster session presented at: 10th European Glaucoma Society Congress; 17\u201322 June 2012 Copenhagen Denmark: European Glaucoma Society 2012."},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2010.03.012"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2004.052795"}],"container-title":["Clinical &amp; Experimental Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fceo.13172","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ceo.13172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T15:01:22Z","timestamp":1694876482000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ceo.13172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,25]]},"references-count":14,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["10.1111\/ceo.13172"],"URL":"https:\/\/doi.org\/10.1111\/ceo.13172","archive":["Portico"],"relation":{},"ISSN":["1442-6404","1442-9071"],"issn-type":[{"type":"print","value":"1442-6404"},{"type":"electronic","value":"1442-9071"}],"subject":[],"published":{"date-parts":[[2018,3,25]]},"assertion":[{"value":"2017-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}